Anti-CD83 Rabbit Monoclonal Antibody for WB, IF-P, ELISA - Q01151

Core Product Specifications and Parameters

Biological Background: CD83 Function and Localization

• CD83 is a transmembrane glycoprotein predominantly expressed on the surface of various immune cells, including dendritic cells and lymphocytes.
• It plays an essential role in CD4+ T-cell selection, differentiation, and stability by regulating the activity of the E3 ubiquitin ligase MARCHF8, which controls MHCII trafficking.
• CD83 inhibits MARCHF1-mediated ubiquitination of MHCII and CD86, preventing their degradation and supporting antigen presentation (PubMed:21220452). Related references: PMID:21220452
• It acts as an important modulator of protective responses against acute infections.
• The protein is localized to the cell membrane and contains an immunoglobulin domain, disulfide bonds, and glycosylation sites.
• CD83 is expressed by activated lymphocytes, Langerhans cells, and activated dendritic cells, reflecting its role in immune activation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (aa 1-100); consider validating specificity in lysates or fixed cells from activated immune cells.
• For western blot (WB), use appropriate loading controls and validate band size (~23 kDa) in relevant species (human, mouse, rat).
• In immunofluorescence (IF-P), optimize fixation and permeabilization conditions for membrane protein detection.
• For ELISA, consider pairing with a detection antibody that recognizes a distinct epitope to avoid competition.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-CD83 monoclonal antibody supports research into immune regulation, dendritic cell biology, and T-cell development. As your sourcing partner, we offer validated antibody pairs, bulk ancillary reagents, and customized rare target raw-material sourcing.