Anti-CD83 Rabbit Monoclonal Antibody for WB, ELISA - Q01151

Core Product Specifications and Parameters

Biological Background: CD83 Function and Localization

• CD83 is a transmembrane glycoprotein predominantly expressed on immune cells such as dendritic cells and lymphocytes. It is also known as B-cell activation protein and cell surface protein HB15.
• It plays an essential role in CD4+ T-cell selection, differentiation, and stability by regulating the activity of MARCHF8, the major E3 ubiquitin ligase controlling MHCII trafficking. Related references: PMID:21220452
• CD83 inhibits MARCHF1 association with MHCII or CD86, preventing their ubiquitination and subsequent degradation. Related references: PMID:21220452
• It acts as an important modulator of protective responses against acute infections.
• Subcellular localization: CD83 is a membrane protein.
• It is specifically expressed in activated lymphocytes, Langerhans cells, and activated dendritic cells.
• The protein features a signal peptide, an immunoglobulin domain, disulfide bonds, and a transmembrane helix. It is a glycoprotein identified by direct protein sequencing and proteomics.
• The predicted molecular weight of CD83 is approximately 23 kDa (23042 Da), consistent with its glycosylated transmembrane nature.

Experimental Guidance and Technical Tips

• The antibody was generated against a recombinant protein spanning the extracellular domain (amino acids 20-143) of human CD83, suggesting it may recognize the native protein in membrane fractions or whole cell lysates.
• For Western blot, anticipate a band around 23 kDa; consider optimizing lysis and reducing conditions.
• For ELISA, this antibody may serve as a capture or detection reagent; validate performance in your specific sample system.
• As a rabbit monoclonal antibody, it is expected to offer high specificity and lot-to-lot consistency; confirm with appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutes a streamlined one-stop sourcing solution for IVD raw materials, supporting every stage from concept to clinical use. Our CD83 antibody portfolio addresses immune cell biology and dendritic cell research needs. We provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your diagnostic development.