Anti-CD81 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P60033

Core Product Specifications and Parameters

Biological Background: CD81 Function and Localization

• CD81, also known as TAPA-1 or Tspan-28, is a 26 kDa tetraspanin family protein that organizes tetraspanin-enriched microdomains (TERMs) critical for receptor clustering and signaling.
• In B cells, CD81 is essential for CD19 surface expression and BCR signal transduction, lowering the threshold for antigen-triggered activation and antibody production.
  Related references: PMID:15161911, PMID:20237408, PMID:27881302
• In T cells, it facilitates CD3 zeta localization at synaptic junctions and promotes Th2 polarization.
  Related references: PMID:22307619, PMID:23858057, PMID:8766544
• CD81 acts as a receptor for hepatitis C virus (HCV) entry into hepatocytes and is also exploited by Plasmodium falciparum sporozoites for liver infection.
• It regulates homotypic and heterotypic cell fusion: inhibits macrophage multinucleation and myotube formation, while positively modulating sperm-egg fusion.
  Related references: PMID:12796480
• Localized to the cell membrane and basolateral membrane; expressed in B cells, hepatocytes, macrophages, and T cells.
  Related references: PMID:20237408, PMID:12483205, PMID:12796480, PMID:22307619
• Contains multiple transmembrane helices, a disulfide bond, and lipid-binding motifs; involved in host-virus interaction and adaptive immunity.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein corresponding to amino acids 114-199 of human CD81, a region likely exposed on the extracellular loop, which may yield antibodies recognizing native conformation. Consider using this antibody in applications requiring detection of endogenous CD81.
• For Western blot, a standard reducing SDS-PAGE with a predicted molecular weight of 26 kDa is expected; however, glycosylation or other post-translational modifications may cause band shifts. Validate detection in your relevant cell lysates.
• For immunohistochemistry (IHC-P), antigen retrieval with citrate buffer pH 6.0 and detection with chromogenic substrates can be tested; positive staining is anticipated in tissues like lymphoid organs and liver.
• In immunofluorescence (IF/ICC), permeabilization with 0.1% Triton X-100 and blocking with serum-matched to the secondary antibody may improve signal-to-noise. CD81 membrane localization should appear punctate or diffuse on the cell surface.
• ELISA applications can be developed with this polyclonal antibody as a capture or detection reagent; pair it with a monoclonal anti-CD81 for sandwich assays if needed.
• Cross-reactivity with mouse and rat indicates epitope conservation; always confirm specificity in your experimental system using appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with reliable access to high-quality IVD raw materials, including validated antibodies like this anti-CD81 polyclonal antibody. Specializing in immunology, infectious disease, and cell biology targets, we supply optimized monoclonals, polyclonals, and antibody pairs to accelerate assay development. Whether you need bulk ancillary reagents or hard-to-find raw materials, CamelBio bridges your sourcing gaps from concept to clinic.