Anti-CD80/B7-1 Monoclonal Antibody for Flow Cytometry - P33681

Core Product Specifications and Parameters

Biological Background: CD80 Function and Localization

• CD80, also known as B7-1, is a member of the immunoglobulin superfamily and a key costimulatory molecule expressed on antigen‑presenting cells.
• It provides the primary costimulatory signal for naïve T‑cell activation by binding CD28, augmenting TCR/MHC engagement.
  Related references: PMID:12196291
• CD28/CD80 ligation activates NF‑κB and MAPK pathways, leading to cytokine production and T‑cell proliferation.
  Related references: PMID:10438913
• CD80 also interacts with CTLA4, a high‑affinity inhibitory receptor, to downregulate T‑cell responses and maintain immune tolerance.
  Related references: PMID:10583602 PMID:11279502
• The CD28/CD80 axis enhances T‑cell metabolic fitness by stimulating glucose uptake and ATP synthesis via PI3K/Akt signaling.
  Related references: PMID:12121659
• CD80 regulates PD‑L1/PD‑1 checkpoint interactions to prevent excessive immunosuppression.
  Related references: PMID:36727298
• Expressed on B‑cells, CD80 drives germinal center reactions and humoral immunity by facilitating T–B cell cross‑talk.
• The protein is a transmembrane glycoprotein localized to the cell membrane, with post‑translational modifications including disulfide bonds, palmitoylation, and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (amino acids 35–242), covering the ligand‑binding region; the antibody is therefore suitable for detecting both cell‑surface and soluble CD80 in flow cytometry.
• For optimal signal‑to‑noise, titrate the antibody and include appropriate isotype and fluorescence minus‑one controls.
• Fixation/permeabilization steps are not required for surface staining, but may be needed if monitoring internalized receptor pools.
• This antibody has demonstrated reactivity with human CD80; for cross‑species applications, validate in the target sample system.
• To preserve fluorophore performance, protect the vial from light during handling and storage.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research institutions to supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents that support robust assay development. This CD80 monoclonal antibody enables immunophenotyping and immune checkpoint analyses in flow cytometry, accelerating your immunology‑focused projects. For specialized rare‑target raw materials or custom sourcing needs, our global procurement and technical teams are ready to assist.