Anti-CD80 Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P33681

Core Product Specifications and Parameters

Biological Background: CD80 Function and Localization

• CD80 (B7-1) is a type I transmembrane glycoprotein of the immunoglobulin superfamily (IgSF) expressed primarily on antigen-presenting cells (APCs) such as dendritic cells, macrophages, and activated B cells.
• It binds CD28 on resting T cells to deliver a critical costimulatory signal that, together with TCR/MHC engagement, triggers T-cell activation, proliferation, and survival via NF-κB and MAPK pathways. Related references: PMID:12196291, PMID:10438913
• CD80 also interacts with CTLA4 (CD152), an inhibitory receptor, to downregulate T-cell responses and maintain peripheral tolerance. Related references: PMID:10583602, PMID:11279502
• The CD28–CD80 axis enhances T-cell metabolic activity by stimulating glucose metabolism and ATP production via PI3K/Akt signaling. Related references: PMID:12121659
• CD80 regulates the PD‑L1/PD‑1 immune checkpoint by limiting excessive PD‑L1 engagement, thereby balancing co‑stimulatory and co‑inhibitory signals. Related references: PMID:36727298
• On B cells, CD80 is essential for germinal center formation, T-cell help, and the generation of high‑affinity antibody responses. (By similarity)
• It serves as a host cell receptor for adenovirus subgroup B, mediating viral attachment and entry.
• The protein undergoes extensive post‑translational modifications, including N‑linked glycosylation, disulfide bond formation, palmitoylation, and phosphorylation, which modulate its stability and signaling capacity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N‑terminal extracellular region (amino acids 1–100) of human CD80. The polyclonal antibody is expected to recognize the mature protein on the cell surface.
• For Western blot (WB), use standard reducing and denaturing conditions. The predicted molecular weight is ~33 kDa, but N‑linked glycosylation often increases the apparent mass. Validate optimal protein load and exposure time in your sample system.
• For immunofluorescence (IF‑P) and immunohistochemistry (IHC‑P), standard fixation and antigen retrieval protocols are recommended. Test the antibody on both fresh‑frozen and formalin‑fixed paraffin‑embedded tissue sections to establish optimal conditions.
• In ELISA, the purified polyclonal antibody can be employed as capture or detection reagent. Perform checkerboard titrations to determine the best working concentrations for your assay format.
• The supplier reports cross‑reactivity with mouse and rat CD80. Confirm reactivity in your species and application of interest, as sequence homology within the immunogen region may influence performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one‑stop partner for IVD raw material sourcing, offering a comprehensive portfolio of validated antibodies, antigen‑matched antibody pairs, and ancillary reagents for immunoassay development. This anti‑CD80 polyclonal antibody is part of our growing immunology catalog, supporting researchers and manufacturers in flow cytometry, immunohistochemistry, and ELISA‑based diagnostic applications. Beyond catalog products, we provide custom antibody development, bulk production, and hard‑to‑find target sourcing to accelerate your assay development from concept to clinical application.