Anti-CD80 Rabbit Monoclonal Antibody for WB, FC, ELISA - Q00609

Core Product Specifications and Parameters

Biological Background: CD80 Function and Localization

• CD80 (B7-1) is a costimulatory molecule belonging to the immunoglobulin superfamily that plays a critical role in T-lymphocyte activation. Related references: PMID:16339518, PMID:1714935
• CD80 acts as a ligand for CD28, providing a primary costimulatory signal that augments MHC/TCR signaling in naive T-cells, leading to activation of NF-kappa-B and MAPK pathways. Related references: PMID:16339518
• CD80 also binds CTLA4, acting as an inhibitor of T-cell activation by blocking CD28-mediated priming.
• On B-cells, CD80 regulates interactions with T-cells in both early and late germinal center responses, crucial for humoral immunity. Related references: PMID:22450810
• CD80 localizes to the cell membrane as a single-pass type I membrane protein.
• Tissue expression includes activated B-cells, gamma interferon-stimulated monocytes, and non-circulating B-cell malignancies.
• Post-translational modifications include alternative splicing, disulfide bond formation, and glycosylation.
• CD80 also regulates PD-L1/PD-1 interactions to limit excessive inhibitory signaling.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 38-245 of mouse CD80) spans the extracellular domain; consider using this antibody to detect both membrane and soluble CD80.
• For western blotting, test reducing and non-reducing conditions due to the disulfide-bonded structure of CD80.
• In flow cytometry, validate the antibody on activated versus resting immune cells to confirm specific CD80 detection.
• Cross-reactivity with human CD80 makes this antibody suitable for cross-species studies; however, epitope conservation should be verified.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a leading one-stop partner for IVD raw materials, technical services, and consultative support, guiding projects from concept to clinic. For CD80 and immune checkpoint research, we supply validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents to streamline your immunoassay development. Our global network also enables rapid sourcing of rare-target antibodies and antigens to meet your diagnostic or research needs.