Anti-CD80 Rabbit Monoclonal Antibody for WB, FC, ELISA - P33681

Core Product Specifications and Parameters

Biological Background: CD80 Function and Localization

• CD80 (B7-1, BB1) is a T-lymphocyte activation antigen belonging to the immunoglobulin superfamily, playing a pivotal role as a costimulatory molecule in T-cell activation (PubMed:38467718).
  Related references:
  PMID:38467718

• It serves as a ligand for the stimulatory CD28 receptor on naive T cells, providing the essential secondary signal for activation, and also binds the inhibitory CTLA-4 receptor, which acts as a decoy to block CD28-mediated priming (PubMed:12196291, PubMed:10583602, PubMed:11279502).
  Related references:
  PMID:12196291, PMID:10583602, PMID:11279502

• Downstream signaling through CD28/CD80 activates NF-kappa-B and MAPK pathways, leading to cytokine production, and additionally stimulates glucose metabolism and ATP synthesis via the PI3K/Akt pathway (PubMed:10438913, PubMed:12121659).
  Related references:
  PMID:10438913, PMID:12121659

• CD80 also modulates PD-L1/PD-1 interactions to limit excessive inhibitory signals and plays a critical role in germinal center reactions by mediating B-cell–T-cell crosstalk for effective humoral immunity (PubMed:36727298) (By similarity).
  Related references:
  PMID:36727298

• This transmembrane glycoprotein is localized on the cell membrane and expressed primarily on antigen-presenting cells, including B cells, dendritic cells, and macrophages.

• Post-translational modifications include glycosylation, phosphorylation, and palmitoylation; the protein features a signal peptide, an immunoglobulin V-type domain, and a transmembrane helix.

• Additionally, CD80 can serve as a host cell receptor for adenovirus subgroup B during infection.

• Multiple isoforms are generated through alternative splicing, contributing to functional diversity.

Experimental Guidance and Technical Tips

• For Western Blot, CD80 may appear as multiple bands ranging from 18 to 33 kDa due to post-translational modifications such as glycosylation; consider running both reducing and non-reducing gels to resolve the specific band.
• In Flow Cytometry, since CD80 is a cell surface protein, we recommend staining live, unfixed cells; titrate the antibody to determine the optimal concentration for your experimental system.
• For ELISA applications, the antibody can be utilized as either a capture or detection reagent; validate its performance with your specific antigen and buffer conditions.
• As this is a recombinant rabbit monoclonal antibody, it demonstrates high batch-to-batch consistency and is ideal for reproducible quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated monoclonal antibodies, antibody pairs, and bulk ancillary reagents, supporting diagnostic manufacturers and research laboratories from concept to clinical application. For immunology and immuno-oncology research, we can supply optimized CD80 antibodies and related immune checkpoint reagents tailored to your development needs. Contact us to explore how our one-stop sourcing solutions can accelerate your project.