Anti-CD79B Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P40259

Core Product Specifications and Parameters

Biological Background: CD79B Function and Localization

• CD79B, also known as B-cell antigen receptor complex-associated protein beta chain, Ig-beta, or B29, is a transmembrane protein that forms a heterodimer with CD79A to constitute the signaling component of the B-cell receptor (BCR) complex.
• It is essential for BCR signal transduction, leading to internalization of the complex, trafficking to late endosomes, and antigen presentation, thereby coupling antigen recognition to downstream signaling cascades.
• CD79B enhances phosphorylation of CD79A, possibly by recruiting kinases or protecting CD79A from dephosphorylation, thus amplifying BCR signaling.
• The protein is specifically expressed in B cells, consistent with its role in B-cell immunity.
• CD79B is localized to the cell membrane as a single-pass type I membrane protein with an extracellular immunoglobulin-like domain, a transmembrane helix, and a cytoplasmic tail containing immunoreceptor tyrosine-based activation motifs (ITAMs) involved in signal transduction.
• Post-translational modifications include glycosylation, disulfide bond formation, and phosphorylation; it is also subject to ubiquitination.
• Alternative splicing and disease variants are noted, with mutations associated with agammaglobulinemia and other immunodeficiencies.
• The protein has a molecular weight of approximately 26 kDa (calculated 26048 Da) and is highly conserved across species, supporting its detection in human, mouse, and rat.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 130-229 of human CD79B, covering the extracellular domain. This likely generates antibodies recognizing the extracellular region, suitable for detecting cell surface CD79B in flow cytometry or IHC-P on fixed tissues; however, validation in these applications would be required.
• For Western blotting, the predicted band is ~26-30 kDa (depending on glycosylation). We recommend using a positive control such as Raji or Daudi B-cell lysates and confirming specificity with CD79B knockdown or knockout if possible.
• In IHC-P, antigen retrieval conditions should be optimized; membranous staining in B-cell areas of lymphoid tissues (e.g., tonsil, spleen) is expected.
• For ELISA, the antibody can be used as a capture or detection reagent when paired with another CD79B antibody, but pairwise testing is necessary to avoid competition.
• Cross-reactivity with mouse and rat is indicated, but due to sequence homology, verify performance in your specific model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw materials, supporting diagnostic manufacturers and researchers from concept to clinic. This anti-CD79B rabbit polyclonal antibody exemplifies our commitment to providing high-quality reagents for B-cell immunology and hematologic malignancy research. Beyond individual antibodies, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target material sourcing to streamline your assay development and production.