Anti-CD79b Rabbit Monoclonal Antibody (B29, Ig-beta) for WB, IF, FC, ELISA - P15530

Core Product Specifications and Parameters

Biological Background: CD79b Function and Localization

• CD79b, also known as B29 or Ig-beta, is the beta chain of the B-cell antigen receptor (BCR) complex, essential for B-cell development and function.
• It cooperates with CD79A to initiate signal transduction upon BCR activation, leading to internalization, trafficking to late endosomes, and antigen presentation.
• CD79b enhances phosphorylation of CD79A, likely by recruiting kinases or protecting CD79A from dephosphorylation, amplifying BCR signaling.
• The protein is located on the cell membrane of B-cells, consistent with its role in antigen recognition and signaling.
• It contains an immunoglobulin domain and a transmembrane helix, and undergoes post-translational modifications including disulfide bond formation, glycosylation, and phosphorylation.
• Keywords: Adaptive immunity, cell membrane, glycoprotein, phosphoprotein, receptor, transmembrane, Ubl conjugation; highlighting its dynamic regulation.

Experimental Guidance and Technical Tips

• This rabbit monoclonal antibody was generated using a recombinant fusion protein covering the extracellular domain (aa 26-158), which includes the immunoglobulin domain, likely recognizing the native conformation.
• For Western blot, a band around 25 kDa is expected; consider using reducing conditions as CD79b forms disulfide-linked heterodimers with CD79A.
• In flow cytometry and immunofluorescence, verify membrane staining on B-cell populations; include appropriate isotype controls and validate in mouse and rat samples.
• For ELISA, the antibody may be paired with a detection reagent specific for mouse CD79b; titration is recommended for optimal signal-to-noise ratio.
• Pre-testing in the relevant sample matrix is advised to confirm performance; antigen retrieval may not be necessary for IF on frozen sections.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This CD79b antibody supports B-cell research and immunoassay development. Explore our portfolio of validated antibody pairs, bulk ancillary reagents, and rare target sourcing to accelerate your project.