Anti-CD79B Monoclonal Antibody for WB, IHC-P, ELISA - P40259

Core Product Specifications and Parameters

Biological Background: CD79B Function and Localization

• CD79B encodes the B-cell antigen receptor complex-associated protein beta chain, also known as Ig-beta or B29, a type I transmembrane protein essential for adaptive immunity.
• It forms a heterodimer with CD79A; together they are required for B-cell receptor (BCR) signal transduction, leading to internalization of the complex, trafficking to late endosomes, and antigen presentation.
• CD79B enhances the phosphorylation of CD79A, likely by recruiting tyrosine kinases or protecting CD79A from dephosphorylation, thereby amplifying BCR signaling.
• The protein is specifically expressed on the cell membrane of B lymphocytes, consistent with its role in antigen recognition and immune activation.
• Post-translational modifications include N-linked glycosylation and phosphorylation; the extracellular region contains an immunoglobulin domain and a disulfide bond.
• Multiple isoforms exist due to alternative splicing, and mutations in CD79B are linked to disease variants (keyword: Disease variant).

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 130–229 of human CD79B, corresponding to the cytoplasmic tail; this region may enable detection of both unphosphorylated and phosphorylated forms in permeabilized samples.
• For Western blot, a band near 26 kDa is expected in human and mouse B-cell lysates; confirm specificity using CD79B‑deficient cells or siRNA knockdown.
• In IHC‑P, antigen retrieval conditions should be optimized, and strong membranous staining is anticipated in B‑cell zones of lymphoid tissues (e.g., spleen, lymph node).
• For ELISA, consider coating with the immunizing peptide or recombinant CD79B extracellular domain; perform cross‑checking of binding specificity under native versus denaturing conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a one-stop sourcing bridge for IVD raw materials, streamlining your B‑cell biology and immune signaling research. From validated CD79B antibody pairs and optimized monoclonal antibodies to bulk ancillary reagents and rare‑target sourcing, we support your diagnostic development pipeline. Partner with CamelBio to secure production‑grade raw materials with end‑to‑end supply chain efficiency.