Anti-CD79B Monoclonal Antibody for WB, IF/ICC, FC, ELISA - P40259

Core Product Specifications and Parameters

Biological Background: CD79B Function and Localization

• CD79B, also known as Ig-beta or B29, is the beta chain of the B-cell antigen receptor complex, essential for B-cell development and function.
• In cooperation with CD79A, CD79B initiates signal transduction downstream of the B-cell receptor (BCR), leading to internalization, trafficking to late endosomes, and antigen presentation.
• CD79B is a transmembrane protein localized at the cell membrane, primarily expressed in B cells.
• It contains an immunoglobulin domain and undergoes alternative splicing, yielding isoforms that may contribute to functional diversity.
• Post-translational modifications include disulfide bond formation, glycosylation, phosphorylation, and ubiquitin conjugation, which regulate its activity and stability.
• Mutations in CD79B have been associated with human diseases, including B-cell immunodeficiencies and lymphomas, highlighting its role in adaptive immunity.
• The protein has a predicted molecular weight of approximately 26 kDa (UniProt: 26048 Da), though the antibody detects bands at 14 kDa and 26 kDa, likely corresponding to different forms or fragments.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain of human CD79B (aa 29-159). This region includes the immunoglobulin-like domain, suggesting the antibody recognizes an epitope accessible on the cell surface.
• For western blot, consider using lysates from human B-cell lines or tissues; the antibody may detect bands at ~14 kDa (unglycosylated/core protein) and ~26 kDa (glycosylated mature form) based on provided data.
• For flow cytometry and IF/ICC, the antibody may be compatible with live-cell staining given the extracellular epitope; always include appropriate controls.
• For ELISA, pairing with an anti-CD79B capture antibody targeting a different epitope may yield optimal sensitivity.
• Ensure validation in your specific sample system, as expression levels may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a one-stop sourcing solution for high-quality IVD raw materials. This anti-CD79B monoclonal antibody is part of our extensive portfolio of immunology reagents, and we offer bulk ordering, customizable antibody pairs, and access to a broad range of ancillary reagents to accelerate your B-cell research and diagnostic assay development. Contact us to discuss your specific procurement needs.