Anti-CD79a Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P11912

Core Product Specifications and Parameters

Biological Background: CD79a Function and Localization

• CD79a, also known as Ig-alpha, MB-1 membrane glycoprotein, or CD79a, is the B-cell antigen receptor complex-associated protein alpha chain.
• It is required together with CD79B for the initiation of signal transduction following antigen binding to the B-cell receptor (BCR), leading to internalization, trafficking to late endosomes, and antigen presentation.
• CD79a is essential for BCR surface expression and for efficient differentiation of pro- and pre-B-cells.
• It stimulates SYK autophosphorylation and activation, and binds BLNK to bring it into proximity with SYK for phosphorylation, thereby mediating downstream signaling.
• CD79a also interacts with and enhances the activity of some Src-family tyrosine kinases and represses BCR signaling during immature B-cell development.
• The protein localizes to the cell membrane and is specifically expressed in B-cells.
• Key structural features include an immunoglobulin domain, transmembrane helix, disulfide bonds, glycosylation, and phosphorylation sites.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100), likely targeting an extracellular epitope; consider this when designing detection strategies.
• For western blot, a band around 25 kDa is predicted, but glycosylation may result in a higher apparent molecular weight; use reducing conditions and validate with a known B-cell lysate.
• For immunohistochemistry (IHC-P), test on human tonsil or lymph node sections where B-cells are abundant; optimize antigen retrieval and antibody dilution.
• In ELISA, confirm coating conditions and use recombinant CD79a or B-cell extracts to verify specificity; the antibody may also work in immunoprecipitation given its monoclonal nature.
• To ensure specificity, validate with CD79a-positive and negative cell lines; note that the antibody is cross-reactive with human CD79a only.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your one-stop sourcing partner for IVD raw materials, connecting you with high-quality antibodies like this anti-CD79a rabbit mAb for B-cell lineage marker detection assays. We offer comprehensive supply chains including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and customized sourcing of rare target raw materials to accelerate your diagnostic development from concept to clinic.