Anti-CD79a Monoclonal Antibody for WB, IF/ICC, IF-P, IHC-P, ELISA - P11912

Core Product Specifications and Parameters

Biological Background: CD79a Function and Localization

• CD79a (also known as Ig-alpha, MB-1) is the alpha chain of the B-cell antigen receptor complex, essential for BCR surface expression and signaling.
• It cooperates with CD79B to initiate signal transduction upon antigen binding, leading to internalization of the BCR complex, trafficking to late endosomes, and subsequent antigen presentation.
• CD79a plays a critical role in early B-cell development by promoting efficient differentiation of pro- and pre-B-cells and by stimulating SYK autophosphorylation and activation.
• The protein contains an immunoglobulin-like domain and localizes to the cell membrane, where it functions as a key receptor component in adaptive immunity.
• Post-translational modifications include phosphorylation, glycosylation, methylation, and ubiquitination, which regulate its signaling activity and stability.
• Expression is specific to B-cells, making it a reliable marker for B-cell lineage studies and diagnostics.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 26-226) of human CD79a, which is well-conserved across species, contributing to cross-reactivity with mouse and rat.
• For western blot (WB), a band at approximately 25 kDa is expected; consider using reducing conditions and validating with B-cell lysates.
• For immunohistochemistry (IHC) and immunofluorescence (IF), optimize antigen retrieval and include appropriate B-cell positive control tissues (e.g., tonsil, lymph node) and negative controls.
• This antibody can be used in ELISA for detecting CD79a in serum or cell culture supernatants; pairing with a matched detection antibody may enhance assay sensitivity.
• Since CD79a is primarily expressed in B-cells, ensure sample enrichment or positive selection for B-cell populations when working with heterogeneous samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for diagnostic manufacturers and research laboratories, providing a wide range of high-quality IVD raw materials including validated antibody pairs, recombinant proteins, and ancillary reagents. For B-cell immunology studies, we offer optimized monoclonal antibodies like this CD79a antibody alongside complementary detection tools and bulk supply options. Our expertise in rare target sourcing and technical consultation supports seamless integration from assay development to clinical application.