Anti-CD74 Rabbit PolymAb® - P04233-2 / P04441-2

Core Product Specifications and Parameters

Biological Background: CD74 Function and Localization

• The CD74 protein is also designated as the HLA class II histocompatibility antigen gamma chain, invariant chain (Ii), and HLA-DR antigens-associated invariant chain.
• It is encoded by the CD74 gene (alternative symbol: DHLAG) in humans.
• As the invariant chain, CD74 associates with MHC class II molecules (HLA-DR antigens) and regulates their assembly and transport.
• This product targets isoform p35 (UniProt accession P04233-2), which has a calculated molecular mass of approximately 26.4 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 73-232 of human CD74 (NP_004346.1), spanning the extracellular region. This design supports the detection of multiple isoforms.
• In western blotting, the antibody detects bands at approximately 24 and 26 kDa, consistent with the p33 and p35 isoforms.
• For immunofluorescence and immunohistochemistry, consider optimizing fixation/permeabilization steps and antigen retrieval methods based on tissue type.
• Use appropriate controls such as B-lymphoblastoid cell lines or human tonsil/spleen tissue for positive staining.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material supplier, offering a wide range of products to accelerate CD74-related diagnostic and research applications. We provide validated monoclonal antibodies, recombinant proteins, and ancillary reagents under one roof, reducing your sourcing effort and lead times. Partner with us to streamline the procurement of high-quality raw materials, from early-stage assay development to clinical manufacturing.