Anti-CD74 Monoclonal Antibody for WB, IHC-P, ELISA - P04233

Core Product Specifications and Parameters

Biological Background: CD74 Function and Localization

• CD74, also known as HLA class II histocompatibility antigen gamma chain or invariant chain (Ii), is encoded by the CD74 gene (DHLAG) and functions as a critical chaperone in MHC class II antigen processing and presentation.
• It binds to peptide-free MHC class II alpha/beta heterodimers, forming a stable alpha-beta-CLIP complex that prevents premature peptide loading, ensuring proper transport from the endoplasmic reticulum to the endosomal/lysosomal system where antigen loading occurs.
• CD74 acts as a chaperone for cathepsin L (CTSL), stabilizing the mature enzyme in endocytic compartments and extracellular spaces of antigen-presenting cells (APCs).
• The p41 isoform exhibits direct antiviral activity by blocking endosomal entry of Ebola virus and coronaviruses, including SARS-CoV-2, through disruption of cathepsin-mediated viral glycoprotein processing (PubMed:32855215).
  Related references:
  PMID:32855215
• CD74 is localized to the cell membrane, endoplasmic reticulum, Golgi apparatus, trans-Golgi network, endosomes, lysosomes, and is also secreted; its expression is detected in urine and enriched in B cells.
• The protein undergoes various post-translational modifications including alternative initiation, alternative splicing, disulfide bond formation, glycosylation, phosphorylation, and proteoglycan attachment, contributing to its functional diversity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–100) of human CD74; consider that this antibody may recognize both isoforms and cleaved forms containing this epitope.
• For Western blot, a band around 34 kDa (consistent with the full-length protein) is expected; additional bands may appear due to proteolytic processing or alternative isoforms.
• In immunohistochemistry (IHC-P), focus on membrane and intracellular vesicular staining patterns in immune tissues, and validate with appropriate controls.
• ELISA performance should be optimized using recombinant CD74 protein or the synthetic peptide immunogen as a coating antigen.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering this anti-CD74 monoclonal antibody along with validated antibody pairs, bulk ancillary reagents, and support for rare target procurement. With expertise spanning from concept to clinic, we facilitate accelerated development of immune function assays and infectious disease diagnostics.