Anti-CD73/NT5E Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P21589

Core Product Specifications and Parameters

Biological Background: CD73/NT5E Function and Localization

• CD73/NT5E encodes the ecto-5'-nucleotidase, a glycosylphosphatidylinositol (GPI)-anchored membrane protein that catalyzes the hydrolysis of extracellular nucleotide monophosphates to their corresponding nucleosides and inorganic phosphate.
• Catalyzes the hydrolysis of AMP to adenosine and inorganic phosphate, with a preference for ribonucleotide monophosphates over deoxyribose forms (PubMed:21933152, PubMed:22997138, PubMed:23142347, PubMed:34403084). Related references:
• PMID:21933152
• PMID:22997138
• PMID:23142347
• PMID:34403084
• Exhibits broad substrate specificity, including IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD, and NMN (PubMed:21933152, PubMed:22997138, PubMed:23142347). Related references:
• PMID:21933152
• PMID:22997138
• PMID:23142347
• Localized to the cell membrane via a C-terminal GPI anchor, positioning the active site extracellularly to regulate purinergic signaling and immune responses.
• Requires zinc as a cofactor for catalytic activity; the enzyme contains a binuclear zinc center essential for phosphate hydrolysis.
• Post-translational modifications include N-linked glycosylation and disulfide bond formation, contributing to protein stability and function.
• Genetic variants in NT5E are associated with arterial calcification due to deficiency of CD73 (ACDC), a rare autosomal recessive disorder.

Experimental Guidance and Technical Tips

• The immunogen corresponds to residues 27–377 of human NT5E, which spans the majority of the extracellular domain; this design favors detection of cell surface CD73 in its native conformation.
• For Western blotting (WB), consider a dilution range of 1:500–1:2000, and be aware that the predicted molecular weight is ~63 kDa; under reducing conditions, glycosylated forms may appear slightly higher.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), antigen retrieval with citrate buffer (pH 6.0) is often beneficial; validate in a panel of positive and negative control tissues.
• Cross-reactivity with mouse and rat has been demonstrated, but given species-specific sequence variations, optimal working dilutions should be determined empirically for each assay.
• As a polyclonal antibody, batch-to-batch consistency may vary; we recommend titrating each new lot and including appropriate positive and negative controls in every experiment.
• For ELISA, direct coating of the recombinant protein or capture-detection formats may be tested; perform checkerboard titrations to optimize the signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports IVD manufacturers and research labs with a comprehensive supply chain of high-quality raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents. The CD73/NT5E polyclonal antibody fits into workflows investigating purinergic signaling, adenosine metabolism, and immune regulation. Whether you require bulk quantities, customized conjugations, or are struggling to source rare-target reagents, contact CamelBio to discuss how we can streamline your procurement from concept to clinic.