Anti-CD73/NT5E Polyclonal Antibody for WB, IHC-P, and ELISA - P21589

Core Product Specifications and Parameters

Biological Background: CD73/NT5E Function and Localization

• CD73/NT5E encodes the ecto-5'-nucleotidase enzyme, a glycosylphosphatidylinositol (GPI)-anchored cell membrane protein that catalyzes the hydrolysis of extracellular nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside.
• The enzyme preferentially hydrolyzes AMP, but also accepts IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD, and NMN as substrates. Related references: PMID:21933152, PMID:22997138, PMID:23142347
• CD73 shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms. Related references: PMID:34403084
• The protein is anchored to the outer leaflet of the plasma membrane via a GPI moiety and contains disulfide bonds, N-linked glycosylation sites, and a zinc-binding site that are essential for its structural integrity and catalytic activity.
• Alternative splicing of the NT5E gene produces multiple isoforms, which may differ in their enzymatic properties or subcellular distribution.
• The UniProt record lists 'Disease variant' as a keyword, suggesting that mutations in NT5E may be associated with human diseases.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 32-264), suggesting the antibody can recognize native cell surface CD73 in intact cells and tissues.
• For Western blotting, expect a band near 63 kDa; however, N-linked glycosylation may cause migration to a higher apparent molecular weight; validate in your specific sample system.
• In immunohistochemistry (IHC-P), consider antigen retrieval conditions and include appropriate positive and negative controls, especially in tissues known to express CD73 such as placenta, kidney, and lymphoid organs.
• Cross-reactivity with mouse and rat CD73 is reported; verify expected bands in those species if using non-human samples.
• For ELISA, the antibody can be employed as a capture or detection reagent; pair with a validated monoclonal antibody or another batch for sandwich ELISA optimization.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop IVD raw-material sourcing partner, CamelBio supports diagnostic manufacturers and researchers in advancing CD73-related immunoassays by offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, and ancillary reagents. Our supply capabilities extend to bulk quantities, rare target sourcing, and technical support to help streamline your assay development from concept to commercialization.