Anti-CD72 Rabbit Polyclonal Antibody for WB, ELISA - P21854

Core Product Specifications and Parameters

Biological Background: CD72 Function and Localization

• CD72 is a B-cell differentiation antigen (also known as Lyb-2) that functions as a co-receptor of the B-cell receptor (BCR), playing both positive and negative regulatory roles in B-cell responses.
• It recognizes the Sm/ribonucleoprotein (RNP) self-antigen ligand, and coligation with the BCR inhibits BCR signaling by recruiting the phosphatase PTPN6/SHP-1 to the BCR complex.
• CD72 acts as a ligand for CD5, thereby playing a critical role in maintaining regulatory T-cell and B-cell homeostasis.
• The protein is a type II transmembrane glycoprotein belonging to the C-type lectin-like domain family, with a single transmembrane helix and a short cytoplasmic tail.
• Subcellularly, CD72 is localized to the plasma membrane and is specifically expressed on the surface of pre-B cells and mature B cells, but not on terminally differentiated plasma cells.
• Post-translational modifications include phosphorylation, glycosylation, and disulfide bond formation, which are important for its signaling functions and structural stability.
• CD72 is often used as a pan-B-cell marker and is implicated in autoimmune diseases due to its role in B-cell tolerance and negative regulation of BCR signaling.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 120–359) of human CD72, which may contribute to the antibody's cross-reactivity with mouse and rat orthologs.
• For Western blot, expect a predominant band near 40 kDa; however, glycosylation may cause a shift to a higher apparent molecular weight; consider using reducing conditions and validate with suitable positive controls.
• In ELISA, the antibody may be used for direct or indirect detection; optimal working dilutions should be determined empirically in the relevant assay system.
• Given the cross-reactivity with human, mouse, and rat, this antibody is suitable for comparative studies across species; confirm reactivity in your specific experimental model.
• CD72 expression is restricted to B-cell lines and primary B cells; ensure appropriate cell or tissue lysates are included as positive controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—from concept to clinic. This anti-CD72 polyclonal antibody supports B-cell biology research, including studies on autoimmune diseases and immune regulation. CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare target raw materials to streamline your development pipeline.