Anti-CD72 Monoclonal Antibody for WB, IHC-P, ELISA - P21854

Core Product Specifications and Parameters

Biological Background: CD72 Function and Localization

• B-cell differentiation antigen CD72, also known as Lyb-2, is encoded by the CD72 gene in humans.
• Functions as a co-receptor of the B-cell receptor (BCR), playing both positive and negative regulatory roles in B-cell functions.
• Recognizes the Sm/ribonucleoprotein (RNP) self-antigen ligand; coligation of CD72 with the BCR inhibits BCR signaling through the recruitment of PTPN6/SHP-1 to the BCR complex.
• Serves as a ligand for CD5, contributing to the maintenance of regulatory T and B-cell homeostasis.
• Localizes to the membrane as a single-pass type II membrane protein with a signal-anchor and a transmembrane helix.
• Predominantly expressed on pre-B-cells and B-cells, but absent from terminally differentiated plasma cells.
• Contains a C-type lectin domain and undergoes post-translational modifications including glycosylation and phosphorylation (disulfide bond, glycoprotein, phosphoprotein).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (amino acids 117–359) of human CD72, suggesting the antibody recognizes an epitope in the extracellular domain.
• For Western blot (WB), CD72 runs at an apparent molecular weight of approximately 40 kDa; ensure appropriate membrane blocking and detection conditions.
• In immunohistochemistry (paraffin-embedded IHC-P), consider antigen retrieval methods and validate staining in known positive control tissues such as tonsil or lymph node.
• For ELISA applications, the monoclonal nature of this antibody may allow capture or detection setup; validate in the relevant sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. This CD72 monoclonal antibody facilitates the study of B-cell biology and can serve as a critical component in developing immunoassays for B-cell-related disorders. For your diagnostic development, CamelBio offers one-stop sourcing across the full workflow—from concept to clinic—backed by technical and supply-chain expertise.