Anti-CD71/Transferrin Receptor Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P02786

Core Product Specifications and Parameters

Biological Background: CD71/Transferrin Receptor Function and Localization

• Transferrin receptor protein 1 (TFRC), also known as T9, p90, or CD71, is a transmembrane receptor essential for cellular iron uptake via receptor-mediated endocytosis of transferrin-bound iron. Endosomal acidification releases iron, and the apotransferrin-receptor complex recycles to the cell surface. Related references: PMID:26214738, PMID:41772062
• Positively regulates T and B lymphocyte proliferation by facilitating iron uptake, highlighting its role in immune cell function. Related references: PMID:26642240
• Acts as a lipid sensor: under low dietary stearate (C18:0), TFRC promotes JNK pathway activation, leading to HUWE1-mediated ubiquitination and degradation of mitofusin MFN2, thereby inhibiting mitochondrial fusion. High stearate levels cause TFRC stearoylation and suppress this pathway. Related references: PMID:26214738
• Competes with the hereditary hemochromatosis protein HFE for binding to transferrin at an overlapping C-terminal site, linking it to iron overload disorders.
• Localizes primarily to the cell membrane but also found in melanosomes and can be secreted, as indicated by UniProt keywords and proteomics data.
• Serves as a host cell receptor for multiple viruses, including new-world arenaviruses (Guanarito, Junin, Machupo), rabies virus, SARS-CoV, MERS-CoV, and SARS-CoV-2, hijacking its endocytic pathway for entry. Also acts as a receptor for the malaria parasite Plasmodium vivax.
• Post-translational modifications include disulfide bonds, glycosylation, palmitoylation, and ubiquitination, which regulate its stability, trafficking, and function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-140), which is critical for transferrin binding and may yield antibodies that recognize the extracellular domain. Consider using this antibody for detecting total TFRC in western blotting and immunohistochemistry.
• For immunofluorescence, permeabilization may be necessary to access intracellular epitopes due to the transmembrane nature of the protein; validate in your specific sample system.
• Given the multiple viral entry roles, blocking or detecting TFRC may be useful in infection assays; however, the neutralizing capacity of this polyclonal antibody should be empirically determined.
• The protein weight is approximately 85 kDa, but glycosylation and splice variants may cause slight migration shifts in WB; consider appropriate positive controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For CD71/Transferrin Receptor research, we can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and assist in sourcing rare target raw materials to accelerate your assay development pipelines.