Anti-CD71/Transferrin Receptor Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P02786

Core Product Specifications and Parameters

Biological Background: CD71/Transferrin Receptor Function and Localization

• Transferrin receptor protein 1 (TfR1)/CD71 is the primary mediator of cellular iron uptake. It binds diferric transferrin and internalizes the complex via clathrin-mediated endocytosis. Endosomal acidification releases iron, and the apotransferrin-receptor complex recycles to the cell surface. TfR1 also binds the hereditary hemochromatosis protein HFE at an overlapping C-terminal site. Related references: PMID:26214738 PMID:41772062
• TfR1 positively regulates T and B cell proliferation through iron uptake. Related references: PMID:26642240
• TfR1 acts as a lipid sensor that modulates mitochondrial fusion. Low dietary stearate levels promote JNK pathway activation and HUWE1-mediated ubiquitination of mitofusin MFN2, inhibiting mitochondrial fusion. High stearate levels lead to TFRC stearoylation, inhibiting JNK activation and stabilizing MFN2. Related references: PMID:26214738
• Serves as a host entry factor for several viruses, including new-world arenaviruses (Guanarito, Junin, Machupo), rabies virus, and coronaviruses (SARS-CoV, MERS-CoV, SARS-CoV-2).
• Acts as a receptor for the malaria parasite Plasmodium vivax.
• Predominantly localized at the cell membrane; also found in melanosomes and secreted.
• Post-translationally modified by palmitoylation and ubiquitination; contains disulfide bonds and is glycosylated. It is a disulfide-linked homodimeric type II transmembrane protein with a signal-anchor domain.

Experimental Guidance and Technical Tips

• For Western blotting, expect a band at approximately 85 kDa, corresponding to the glycosylated TfR1 monomer. Use reducing and denaturing conditions.
• In IHC-P, antigen retrieval (heat-induced at pH 6 or 9) can improve staining. Validate on appropriate positive and negative tissue controls.
• For IF/ICC, cell surface TfR1 can be detected without permeabilization; permeabilization enables staining of intracellular pools. Consider live-cell staining for surface expression.
• This rabbit monoclonal antibody was raised against amino acids 100–250 of human TfR1. Cross-reactivity with other species is not reported, so validate in your sample system if used beyond human.
• Validated for ELISA; consider pairing with a non-competing antibody for sandwich assay development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop sourcing partner providing diagnostic manufacturers and labs with a comprehensive portfolio of IVD raw materials, technical services, and consulting—from concept to clinic. For CD71/transferrin receptor research and assay development, we offer validated monoclonal antibodies, matched antibody pairs, and bulk ancillary reagents. Our expertise extends to rare target sourcing and custom services tailored to your iron metabolism, oncology, or infectious disease project needs.