Anti-CD70 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P32970

Core Product Specifications and Parameters

Biological Background: CD70 Function and Localization

• CD70, also known as CD27 ligand (CD27-L) and tumor necrosis factor ligand superfamily member 7 (TNFSF7), is a type II transmembrane protein with a molecular weight of approximately 21 kDa.
• It is expressed at the plasma membrane of B cells and serves as the ligand for the CD27 receptor on T cells, mediating antigen-specific T cell activation and expansion (PubMed:28011863, PubMed:28011864). Related references: PMID:28011863 PMID:28011864
• The CD70-CD27 signaling pathway provides immune surveillance of B cells, linking innate and adaptive immunity.
• CD70 is a member of the tumor necrosis factor superfamily, characterized by a transmembrane helix and a signal-anchor sequence.
• Post-translational modifications include glycosylation and disulfide bond formation, which are critical for its structural integrity and function.
• Alternative splicing of the CD70 gene produces isoforms that may have distinct roles.
• Subcellularly, CD70 localizes to the cell membrane, with its C-terminal TNF homology domain exposed extracellularly to engage CD27.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 47-126 of human CD70 (NP_001243.1), spanning the extracellular domain; therefore, the antibody is expected to recognize the mature, membrane-bound form.
• Validated for Western blot (WB), immunohistochemistry (paraffin-embedded sections, IHC-P), and ELISA; consider pretesting and optimizing conditions for each assay.
• Predicted cross-reactivity with mouse and rat may enable usage in animal models; however, confirm reactivity in relevant sample systems.
• Use appropriate negative and positive controls (e.g., CD70-overexpressing cell lines vs. CD70-deficient cells) to validate specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized rare-target sourcing. This anti-CD70 antibody supports immunology research, particularly the CD70-CD27 signaling axis. Contact us for tailored procurement solutions from concept to clinic.