Anti-CD7 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P09564

Core Product Specifications and Parameters

Biological Background: CD7 Function and Localization

• CD7, also known as GP40, TP41, Tp40, LEU-9, and T-cell surface antigen Leu-9, is a 25 kDa transmembrane glycoprotein that belongs to the immunoglobulin superfamily and contains a single Ig-like domain.
• CD7 is expressed on T-cells and natural killer (NK) cells and their precursors, serving as a key surface marker for lymphoid lineage cells (Tissue Specificity).
• Upon binding to its ligand K12/SECTM1, CD7 provides costimulatory signals that enhance T-cell activation, playing a critical role in adaptive immune responses. Related references: PMID:10652336
• In T-cells, CD7 engagement induces production of cytokines such as IL-2, which promotes proliferation and differentiation of immune effector cells. Related references: PMID:1709867
• On resting NK cells, CD7 activation results in a significant induction of interferon-gamma, highlighting its role in innate-like lymphocyte effector functions. Related references: PMID:7506726
• CD7 is anchored in the plasma membrane as a single-pass type I membrane protein and is post-translationally modified by glycosylation, disulfide bond formation, and palmitoylation (Membrane, Keywords).

Experimental Guidance and Technical Tips

• The immunogen was a synthetic peptide corresponding to amino acids 141-240 of human CD7, which lies within the extracellular region. This region is expected to be accessible in native and denatured protein conformations, making the antibody well-suited for applications targeting the extracellular domain.
• For Western blot (WB), the predicted molecular weight of CD7 is 25 kDa; consider using appropriate molecular weight markers and validate with a positive control such as human T-cell or NK cell lysates.
• In immunohistochemistry-paraffin (IHC-P), ensure proper antigen retrieval and tissue fixation, and validate staining patterns in known CD7-expressing tissues (e.g., lymph node, spleen) to confirm specificity.
• For ELISA, the antibody can be used for direct or indirect detection of recombinant or native CD7; optimal dilution and coating conditions should be experimentally determined.
• Always include appropriate negative and positive controls to confirm antibody specificity and performance in your specific sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated one-stop sourcing partner for IVD raw materials, CamelBio supports diagnostic manufacturers, research institutes, and laboratories with a comprehensive range of products and technical services from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom rare-target raw-material procurement, streamlining development in immunology and T-cell biology. With expertise in reproducible supply chains and rigorous quality, we empower your CD7-targeted assay development with the critical reagents and consultative support you need.