Anti-CD69 Rabbit Monoclonal Antibody for WB, ELISA - Q07108

Core Product Specifications and Parameters

Biological Background: CD69 Function and Localization

• CD69 is a type II transmembrane C-type lectin receptor, also known as AIM, EA1, MLR-3, CLEC2C, GP32/28, BL-AC/P26, and Leu-23. It is rapidly upregulated on the surface of activated T-cells, B-cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhans cells, and platelets.
• It negatively regulates Th17 cell differentiation through carbohydrate-dependent interaction with galectin-1/LGALS1 on immature dendritic cells. Related references: PMID:24752896
• CD69 cooperates with the S100A8/S100A9 complex present on peripheral blood mononuclear cells to promote the conversion of naive CD4 T-cells into regulatory T-cells. Related references: PMID:26296369
• As an oxidized low-density lipoprotein (oxLDL) receptor in CD4 T-lymphocytes, it negatively regulates inflammation by inducing PDCD1 expression through NFAT activation. Related references: PMID:35930205
• In adipose tissue-derived mesenchymal stem cells (ASCs), CD69 specifically recognizes P. aeruginosa and triggers ERK1 activation, leading to granulocyte-macrophage colony-stimulating factor (GM-CSF) and inflammatory cytokine secretion, thus contributing to host defense. Related references: PMID:34841721
• CD69 negatively regulates the chemotactic responses of effector lymphocytes and dendritic cells to sphingosine 1-phosphate (S1P) by acting as a S1PR1 agonist and facilitating receptor internalization and degradation. Related references: PMID:37039481
• The protein resides primarily on the cell membrane as a disulfide-linked homodimer and undergoes post-translational modifications, including glycosylation and phosphorylation.
• It is expressed on various immune cell subsets and plays a role in eosinophil IL-10 production via the ERK1/2 pathway, though further validation in specific model systems is advised.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 62–199 of human CD69 (NP_001772.1), corresponding to the extracellular domain. This region is conserved between human and rat, explaining the observed cross-reactivity.
• For western blot (WB), a band around 23 kDa is expected under reducing conditions; however, due to glycosylation, a higher molecular weight may be observed. Include appropriate positive and negative controls in your experimental design.
• In ELISA applications, consider direct, indirect, or sandwich formats depending on assay requirements. Titrate the antibody to determine the optimal working concentration for your specific protocol.
• As CD69 is transiently expressed upon stimulation, activation of target cells (e.g., with PMA/ionomycin for T-cells) may be necessary to detect the protein. Validate detection conditions in your relevant biological samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutions streamlined access to IVD raw materials, including validated antibodies, recombinant proteins, and ancillary reagents. For immunology and inflammation studies, we provide anti-CD69 monoclonal antibodies and related target reagents backed by a comprehensive sourcing network. Whether you need bulk quantities or rare biomarker raw materials, CamelBio supports your development from concept to clinic.