Anti-CD69 Polyclonal Antibody for WB, ELISA - Q07108

Core Product Specifications and Parameters

Biological Background: CD69 Function and Localization

• CD69, also designated activation inducer molecule (AIM), early T-cell activation antigen p60, or Leu-23, is a transmembrane C-type lectin domain family 2 member C (CLEC2C) encoded by the CD69 gene in humans.
• Serves as an early activation marker rapidly upregulated on the surface of platelets, T-lymphocytes, and NK cells following antigen recognition or cytokine signaling, playing a central role in immune cell homeostasis.
• Negatively regulates Th17 cell differentiation through carbohydrate-dependent interaction with galectin-1 (LGALS1) present on immature dendritic cells, thereby modulating JAK3/STAT5 signaling and RORγt transcription. Related references: PMID:24752896
• Promotes the conversion of naïve CD4 T-cells into regulatory T-cells (Tregs) by engaging the S100A8/S100A9 complex on peripheral blood mononuclear cells (PBMCs). Related references: PMID:26296369
• Functions as an oxidized low-density lipoprotein (oxLDL) receptor on CD4 T-lymphocytes, inducing PDCD1 (PD-1) expression through NFAT activation to limit inflammatory responses. Related references: PMID:35930205
• Contributes to adipose tissue-derived mesenchymal stem cell (ASC)-mediated protection against Pseudomonas aeruginosa infection by recognizing the pathogen and promoting ERK1-dependent GM-CSF secretion. Related references: PMID:34841721
• Acts as a negative regulator of lymphocyte and dendritic cell chemotaxis by serving as an S1PR1 agonist, triggering receptor internalization and degradation, thus reducing responsiveness to sphingosine 1-phosphate (S1P). Related references: PMID:37039481
• Predominantly localized to the cell membrane and expressed on activated T-cells, B-cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhans cells, and platelets. The protein is a glycoprotein and phosphoprotein with intramolecular disulfide bonds.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–80), and as a polyclonal antibody, it recognizes multiple epitopes. Consider validating specificity using activated vs. resting lymphocyte lysates.
• For Western blotting, a band at approximately 23 kDa (under reducing conditions) is expected. Use appropriate positive controls such as PHA-stimulated PBMCs or Jurkat T-cell lysates.
• In ELISA, the antibody may be suitable for detecting soluble CD69 shed from activated cells or for cell-based assays; titration and optimization are recommended.
• Cross-reactivity with mouse and rat has been reported; however, confirm reactivity patterns in your specific experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your IVD raw material procurement with a comprehensive portfolio of validated antibodies, including this CD69 polyclonal antibody for immunology and inflammation research. From optimized monoclonal/polyclonal antibodies and antibody pairs to bulk ancillary reagents, we support every stage of assay development. Our one-stop sourcing model also accommodates rare target requests, ensuring you get the right raw materials efficiently.