Anti-CD69 Monoclonal Antibody for FC - Q07108

Core Product Specifications and Parameters

Biological Background: Early activation antigen CD69 Function and Localization

• CD69 is a transmembrane C-type lectin receptor (CLEC2C) that rapidly upregulates on the surface of platelets, T-lymphocytes, and NK cells upon activation, playing a key role in immune cell homeostasis. Related references: PMID:24752896 PMID:26296369 PMID:35930205
• It negatively regulates Th17 cell differentiation via carbohydrate-dependent interaction with galectin-1 on immature dendritic cells, influencing JAK3/STAT5 signaling and RORγt transcription. Related references: PMID:24752896
• CD69 acts as an oxidized low-density lipoprotein (oxLDL) receptor in CD4 T-cells, inducing PDCD1 expression through NFAT activation to suppress inflammatory responses. Related references: PMID:35930205
• The protein promotes regulatory T-cell conversion from naive CD4 T-cells via the S100A8/S100A9 complex and participates in mesenchymal stem cell‑mediated antibacterial defense by recognizing P. aeruginosa, activating ERK1, and inducing GM-CSF secretion. Related references: PMID:26296369 PMID:34841721
• Subcellularly, CD69 is localized to the cell membrane with a single‑pass transmembrane domain; it is a phosphorylated glycoprotein with disulfide bonds.
• Tissue specificity: expressed on activated T-cells, B-cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhans cells, and platelets.
• CD69 negatively regulates chemotaxis by acting as an S1PR1 agonist, promoting receptor internalization and degradation. Related references: PMID:37039481
• Additional annotations: 3D-structure, direct protein sequencing, proteomics identification, receptor, reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 78‑199 of human CD69, covering part of the extracellular domain; the antibody may recognize native conformational epitopes suitable for flow cytometry.
• Since this is a rabbit monoclonal antibody, consider using directly conjugated or anti‑rabbit secondary reagents for detection.
• Validate the antibody’s performance in relevant activated samples (e.g., human PBMCs stimulated with PMA/ionomycin) and appropriate isotype controls.
• Cross‑reactivity with cynomolgus monkey enables use in non‑human primate translational studies; confirm reactivity in the specific matrix if applied to other assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this high‑quality rabbit anti‑CD69 monoclonal antibody as a vital tool for immunology research and diagnostic development. As your one‑stop IVD raw‑material partner, we provide validated antibody pairs, optimized monoclonals, bulk ancillary reagents, and custom sourcing for rare targets—bridging every stage from concept to clinic.