Anti-CD68 Rabbit PolymAb® for WB, IHC-P, IF/ICC, ELISA - P34810 / P31996

Core Product Specifications and Parameters

Biological Background: CD68 Function and Localization

• Macrosialin (CD68) is a heavily glycosylated transmembrane glycoprotein belonging to the lysosomal-associated membrane protein (LAMP) family, also known as Lamp4, gp110, and Scard1. The core protein has a calculated molecular weight of ~37 kDa, but extensive N- and O-linked glycosylation yields an apparent size of 70–130 kDa on SDS-PAGE.
• CD68 is predominantly expressed on the endosomal and lysosomal membranes of monocytes and tissue macrophages, where it participates in phagocytic and intracellular lysosomal metabolism.
• By binding tissue- and organ-specific lectins or selectins, CD68 facilitates macrophage homing to sites of inflammation or injury and may enable cell crawling over selectin-coated substrates through rapid recycling between endolysosomal compartments and the plasma membrane.
• In addition to macrophages, CD68 is expressed at lower levels in lymphocytes, fibroblasts, endothelial cells, and many tumor cell lines, potentially aiding tumor cell attachment to vascular endothelium and metastatic dissemination.
• Post-translational modifications include disulfide bond formation, heavy glycosylation, and a cleavable signal peptide, contributing to its maturation and membrane trafficking.
• Alternative splicing generates multiple isoforms of CD68, and its functional repertoire is further expanded through interactions with extracellular matrix components and cell adhesion receptors.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein covering both human and mouse CD68, suggesting broad cross-reactivity; nonetheless, researchers should validate reactivity in their specific sample systems.
• For Western blot (WB), a broad band between 70 and 130 kDa is expected due to variable glycosylation; include a positive control such as macrophage lysates and consider using 8–12% SDS-PAGE gels for optimal resolution.
• In IHC-P and IF/ICC, effective detection may require antigen retrieval (heat- or enzyme-based) and permeabilization steps to access the intracellular endosomal/lysosomal localization.
• For ELISA, pairing with a compatible detection antibody is essential; CamelBio offers validated antibody pairs to support rapid assay development.
• Because CD68 is a well-established macrophage marker, double-staining with other lineage markers (e.g., CD163, CD11b) can help delineate macrophage subsets in tissue sections.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, including validated monoclonal and polyclonal antibodies, recombinant proteins, and bulk ancillary reagents. For macrophage biology and immunological profiling, our portfolio of optimized CD68 antibodies and antibody pairs supports robust immunohistochemistry, immunofluorescence, and ELISA kit development. Whether you need large-volume procurement, custom reagent sourcing, or consultation on assay feasibility, CamelBio bridges your workflow from concept to clinical application.