Anti-CD68 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P34810

Core Product Specifications and Parameters

Biological Background: CD68/Macrosialin Function and Localization

• CD68 (macrosialin, Gp110) is a heavily glycosylated type I transmembrane protein that belongs to the lysosome-associated membrane protein (LAMP) family.
• It plays a key role in the phagocytic activities of tissue macrophages, participating in both intracellular lysosomal metabolism and extracellular cell–cell or cell–pathogen interactions.
• CD68 binds to tissue- and organ-specific lectins or selectins, enabling homing of macrophage subsets to particular sites within the body.
• The protein rapidly recycles between endosomes/lysosomes and the plasma membrane, a dynamic process that may facilitate macrophage crawling over selectin-bearing substrates or other cells.
• Subcellularly, CD68 localizes primarily to the cell membrane, endosome membrane, and lysosome membrane.
• It is highly expressed in blood monocytes and tissue macrophages, but can also be found in lymphocytes, fibroblasts, endothelial cells, and many tumor cell lines.
• Post-translational modifications include N-glycosylation and formation of disulfide bonds, which contribute to its structural stability and function.
• Alternative splicing generates multiple isoforms, adding to the functional diversity of CD68.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 22–319, which covers a substantial portion of the extracellular domain; therefore, the antibody is expected to detect both the full-length transmembrane form and any shed or soluble fragments of CD68.
• For immunohistochemistry on paraffin-embedded tissues (IHC-P), consider antigen retrieval (e.g., heat-mediated citrate buffer, pH 6.0) and optimize the primary antibody dilution empirically.
• Cross-reactivity with mouse CD68 has been reported, allowing potential use in mouse models; however, validation in the relevant species and sample type is recommended.
• As a polyclonal antibody, batch-to-batch consistency should be verified for critical quantitative applications, and we recommend including appropriate controls.
• Store the antibody at -20°C upon arrival (shipped on ice bags) and avoid repeated freeze-thaw cycles to maintain performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing bridge for IVD raw materials, providing diagnostic manufacturers, laboratories, and research institutes with streamlined access to high-quality antibodies, validated pairs, and bulk ancillary reagents. Our CD68 rabbit polyclonal antibody supports immunology and macrophage biology research, and we can assist you in sourcing rare-target raw materials or complete assay development suites from concept to clinic. Contact us to explore customized supply solutions tailored to your project needs.