Anti-CD68 Rabbit Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P31996

Core Product Specifications and Parameters

Biological Background: CD68 Function and Localization

• CD68, also known as macrosialin, Lamp4, gp110, or Scard1, is a heavily glycosylated transmembrane protein belonging to the lysosomal-associated membrane protein family.
• It plays a key role in the phagocytic activities of tissue macrophages, facilitating intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions.
• CD68 binds tissue- and organ-specific lectins or selectins, enabling homing of macrophage subsets to specific sites, and its rapid recycling between endosomes, lysosomes, and the plasma membrane may support macrophage migration.
• The protein is predominantly expressed in tissue macrophages and, to a lesser extent, in dendritic cells.
• Subcellularly, CD68 localizes to endosome membranes, lysosome membranes, and the cell membrane.
• Structurally, it features a signal peptide, a transmembrane helix, multiple disulfide bonds, and alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an internal region (amino acids 50–150), which is likely to detect total CD68 in denaturing and native conditions; consider verifying the antibody’s performance in your specific assay.
• For Western blotting, the predicted molecular weight is approximately 35 kDa, but extensive glycosylation may result in a higher apparent molecular weight; validate with relevant positive controls.
• This polyclonal antibody is raised in rabbit against a mouse peptide and cross-reacts with human, mouse, and rat CD68, making it suitable for multi-species studies.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimize tissue fixation and antigen retrieval protocols to ensure robust signal.
• As a polyclonal antibody, batch-to-batch consistency may vary slightly; consider confirming lot-specific performance if transitioning to new production runs.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, laboratories, and research institutes with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies like this anti-CD68 reagent, and bulk ancillary consumables. With expertise across the entire IVD development pipeline, CamelBio supports your pursuit of reliable macrophage biology research tools and can source rare-target raw materials for custom applications. Contact us to streamline your supply chain from concept to clinic.