Anti-CD68 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P34810

Core Product Specifications and Parameters

Biological Background: CD68 Function and Localization

• CD68 is a heavily glycosylated integral membrane protein belonging to the lysosome-associated membrane protein (LAMP) family, also known as macrosialin or Gp110.
• It plays a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions.
• CD68 binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular tissue sites.
• Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
• The protein is localized primarily to endosome and lysosome membranes, with transient expression on the cell membrane.
• Highly expressed in blood monocytes and tissue macrophages, and also found in lymphocytes, fibroblasts, endothelial cells, and many tumor cell lines.
• Its N-terminal mucin-like domain is heavily O-glycosylated, and the protein contains a single transmembrane helix and a short cytoplasmic tail.
• Alternative splicing produces different isoforms, and the protein is classified under keywords including signal peptide, disulfide bond, and glycoprotein.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from amino acids 100–200 of human CD68 (NP_001242.2); consider performing sequence alignment if cross-reactivity with other species is critical.
• For western blotting (WB), note that due to heavy glycosylation, the observed molecular weight may be significantly higher than the calculated 37 kDa; validate with appropriate molecular weight markers.
• In immunofluorescence/immunocytochemistry (IF/ICC), permeabilization is necessary to access the predominantly intracellular epitope.
• ELISA performance should be optimized by titrating the antibody with the relevant recombinant protein or cell lysate.
• Validate the antibody in your specific sample system, as expression levels can vary across tissues and cell lines.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and researchers with high-quality IVD raw materials, including this anti-CD68 polyclonal antibody for macrophage and tumor research. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing to streamline your development workflow. Partner with us for efficient access to critical reagents from discovery to clinical application.