Anti-CD68 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P34810

Core Product Specifications and Parameters

Biological Background: CD68 Function and Localization

• CD68, also known as macrosialin, GP110, LAMP4, or SCARD1, is a heavily glycosylated transmembrane glycoprotein belonging to the LAMP family.
• It is predominantly expressed on the membrane of endosomes and lysosomes, but can also rapidly recycle to the cell surface, suggesting a role in adhesion and cellular motility.
• CD68 functions in the phagocytic activities of macrophages, participating in both intracellular lysosomal metabolism and extracellular cell–cell and cell–pathogen interactions.
• By binding to tissue- and organ-specific lectins or selectins, CD68 facilitates the homing of macrophage subsets to specific anatomical sites.
• The protein contains a mucin-like domain that is likely involved in ligand binding and may mediate attachment to selectins on endothelial cells, aiding in the dissemination of macrophages and tumor cells.
• CD68 is highly expressed in blood monocytes and tissue macrophages, and is also found in lymphocytes, fibroblasts, endothelial cells, and various tumor cell lines.
• Multiple post-translational modifications, including N-linked glycosylation and disulfide bonds, contribute to its structural stability and molecular weight shift to approximately 110 kDa (the mature glycosylated form), although the predicted core protein is about 37 kDa.
• Alternative splicing generates multiple isoforms, which may have distinct functions or expression patterns.

Experimental Guidance and Technical Tips

• The immunogen (recombinant protein corresponding to amino acids 16–200) targets the N-terminal extracellular region; the antibody may recognize both the core protein and the mature glycosylated form.
• For Western blot (WB), it is advisable to test both reducing and non-reducing conditions, and note that the observed band may migrate between 37 kDa (core protein) and 110 kDa (glycosylated form), depending on the sample and experimental conditions.
• For immunofluorescence/immunocytochemistry (IF/ICC), expect a staining pattern consistent with endosomes, lysosomes, and the cell membrane; fixation and permeabilization protocols should be optimized for the cell type.
• ELISA applications may require proper coating and blocking conditions; consider pairing with a suitable detection antibody for sandwich assays.
• Validate the antibody in the relevant species (human, mouse, rat) and appropriate positive control samples, such as macrophage cell lines (e.g., THP-1, RAW 264.7) or peripheral blood mononuclear cells.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated partner for diagnostic manufacturers and research institutions, offering a comprehensive range of IVD raw materials and technical support from concept to clinic. For studies involving macrophage biology, immune cell trafficking, or tumor microenvironment, we supply validated antibody pairs, optimized polyclonal and monoclonal antibodies, and auxiliary reagents for bulk procurement. Our sourcing capabilities also extend to rare targets and custom recombinant proteins, ensuring a seamless supply chain for your CD68-related projects.