Anti-CD68 Rabbit Monoclonal Antibody for WB, IF-P, FC (intra), ELISA - P34810

Core Product Specifications and Parameters

Biological Background: CD68 Function and Localization

• CD68, also known as macrosialin, GP110, LAMP4, and SCARD1, is a heavily glycosylated type I transmembrane protein belonging to the lysosome-associated membrane protein (LAMP) family.
• It is predominantly localized to endosome and lysosome membranes, but can recycle to the cell surface, enabling rapid plasma membrane presentation in macrophages.
• The protein plays a critical role in phagocytosis, mediating intracellular lysosomal metabolism of engulfed material and participating in extracellular interactions with pathogens and other cells.
• CD68 binds to tissue- and organ-specific lectins or selectins, which facilitates homing of macrophage subsets to particular anatomical sites and supports macrophage adhesion and migration.
• Rapid recycling of CD68 from endosomes and lysosomes to the plasma membrane is thought to assist macrophages in crawling over selectin-bearing substrates or other cells.
• Although strongly expressed in blood monocytes and tissue macrophages, CD68 is also detected in lymphocytes, fibroblasts, endothelial cells, and many tumor cell lines, where it may promote tumor cell dissemination by binding to vascular selectins.
• The protein features multiple N-terminal mucin-like domains, a single transmembrane helix, and a short cytoplasmic tail, with extensive N- and O-glycosylation and several intramolecular disulfide bonds.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning residues 100–200 of human CD68 (NP_001242.2). Sequence conservation in this region likely underlies cross-reactivity with mouse and rat.
• For Western blot (WB), the predicted molecular weight of the unglycosylated backbone is ~37 kDa, but heavily glycosylated forms often run at higher apparent molecular weights (80–110 kDa). Appropriate blocking and molecular weight markers are recommended.
• In immunofluorescence (IF-P) and intracellular flow cytometry (FC), permeabilization is essential because CD68 resides primarily in endosomal and lysosomal compartments; surface staining requires consideration of recycling dynamics.
• ELISA performance depends on epitope accessibility; matrix effects and coating conditions should be optimized, as heavy glycosylation may mask or alter antibody binding.
• Given the broad expression in monocyte/macrophage lineages, include relevant positive controls (e.g., THP-1 or RAW 264.7 cells) and isotype controls to confirm specificity in the desired sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw-material sourcing, bridging the gap between research and clinical applications. For CD68-related macrophage, inflammation, and tumor biology studies, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Let CamelBio streamline your raw-material procurement with reliable, one-stop support tailored to your diagnostic development needs.