Anti-CD68 Monoclonal Antibody for WB, IF/ICC, FC, ELISA - P31996

Core Product Specifications and Parameters

Biological Background: CD68 (Macrosialin) Function and Localization

• Macrosialin (CD68), also known as LAMP4, gp110, or SCARD1, is encoded by the Cd68 gene in mouse. Alternative splicing produces multiple isoforms.
• It functions in phagocytic activities of tissue macrophages, including intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions.
• CD68 rapidly recirculates between endosomes/lysosomes and the plasma membrane, which may facilitate macrophage crawling over selectin-bearing substrates.
• The protein is a heavily glycosylated transmembrane glycoprotein with a signal peptide and a disulfide bond, localized mainly to endosome and lysosome membranes, but also detected at the cell membrane.
• Expressed predominantly in tissue macrophages, with lower expression in dendritic cells.
• Through binding to tissue- and organ-specific lectins or selectins, CD68 contributes to macrophage subset homing to particular sites.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 21-291) of mouse CD68, including the extracellular domain, which likely preserves native epitope conformation.
• For western blot, expect a band near the predicted 33-34 kDa; however, extensive glycosylation may shift the apparent molecular weight significantly.
• In immunofluorescence/ICC and flow cytometry, consider that CD68 localizes to endosomal/lysosomal membranes and can be transiently present at the plasma membrane.
• For ELISA, validate assay conditions to account for potential glycosylation-dependent epitope recognition.
• Always validate the antibody’s performance with appropriate positive controls (e.g., mouse macrophage lysates) and negative controls in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your one-stop sourcing bridge for IVD raw materials, offering validated monoclonal antibodies, antibody pairs, and ancillary reagents to diagnostic developers and research labs. This anti-CD68 rabbit monoclonal antibody supports studies in macrophage biology, lysosomal disorders, and immune trafficking. Contact us for bulk orders, custom conjugation, or assistance in sourcing rare targets to accelerate your project from concept to clinic.