Anti-CD66d/CEACAM3 Polyclonal Antibody for WB, ELISA - P40198

Core Product Specifications and Parameters

Biological Background: CD66d/CEACAM3 Function and Localization

• CEACAM3, also designated CD66d or CGM1, is a cell adhesion molecule belonging to the carcinoembryonic antigen (CEA) family and functions as a major granulocyte receptor for CEACAM-binding pathogens.
• It mediates opsonin-independent phagocytosis of microorganisms such as Neisseria, Moraxella, and Haemophilus species, playing a critical role in innate immune clearance.
• The protein is highly granulocyte-specific; its predominant transcript CGM1a is not detected outside the granulocytic lineage, including monocytes, lymphocytes, and a broad panel of tissues.
• CEACAM3 is a transmembrane protein located at the plasma membrane and contains an immunoglobulin domain essential for pathogen recognition.
• Upon pathogen engagement, CEACAM3 stimulates RAC1, a Rho GTPase that triggers actin cytoskeleton rearrangements necessary for phagocytosis.
• According to UniProt, the protein undergoes alternative splicing and is annotated as a glycoprotein and phosphoprotein; its predicted molecular weight is approximately 27 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular region (amino acids 1–135), which includes the immunoglobulin domain; this may allow recognition of the ligand-binding site.
• For Western blot, consider using granulocyte-derived cell lines or primary neutrophil lysates and validate endogenous CEACAM3 expression due to its restricted tissue specificity.
• In ELISA applications, assess reactivity against recombinant CEACAM3 protein and include appropriate negative controls to confirm specificity.
• As a polyclonal antibody, it may display lot-to-lot variability; titration and consistency testing are advisable for quantitative assays.
• The reported cross-reactivity with mouse suggests conserved epitopes; however, optimize assay conditions if switching between species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw-material sourcing, offering this anti-CEACAM3 antibody to support innate immunity and infectious disease assay development. We provide one-stop access to validated antibody pairs, monoclonal and polyclonal reagents, bulk ancillary materials, and technical consulting to streamline your project from concept to clinic. Whether you need off-the-shelf products or custom rare-target sourcing, CamelBio bridges your research needs with reliable supply.