Anti-CD66b/CEACAM8 Polyclonal Antibody for WB, IF/ICC, ELISA - P31997

Core Product Specifications and Parameters

Biological Background: CD66b/CEACAM8 Function and Localization

• CD66b/CEACAM8 (also designated CD67, CGM6, NCA-95) is a GPI-anchored cell surface glycoprotein belonging to the carcinoembryonic antigen (CEA) family.
• It mediates calcium-independent heterophilic cell adhesion, particularly interacting with other CEA family members such as CEACAM6. Related references: PMID:11590190 PMID:2022629 PMID:8776764
• These adhesive interactions are especially important in activated neutrophils, where CEACAM8 engages in heterophilic binding. Related references: PMID:8776764
• Subcellularly, CEACAM8 is localized to the cell membrane and surface.
• Tissue expression is prominent in leukocytes from chronic myeloid leukemia patients and in bone marrow.
• The protein features an immunoglobulin domain, disulfide bonds, and a GPI membrane anchor, with a predicted molecular weight of approximately 38 kDa.

Experimental Guidance and Technical Tips

• For Western blot, expect a band near 38 kDa; appropriate positive controls include neutrophil lysates or CEACAM8-overexpressing cell lines.
• In IF/ICC, optimize permeabilization conditions and antibody concentration to detect cell surface–localized CEACAM8, especially in activated neutrophil samples.
• The immunogen covers amino acids 35–320, representing a large extracellular region, which may facilitate recognition of both native and denatured target conformations.
• As a polyclonal antibody, lot-to-lot performance can vary; validation of each new lot in the intended assay is recommended.

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CamelBio streamlines procurement for IVD developers and research laboratories by providing validated antibodies and ancillary raw materials for immune cell targets such as CD66b/CEACAM8. With expertise in polyclonal antibody sourcing and custom development, CamelBio offers bulk reagents, technical consulting, and supply chain solutions to support projects from early-stage discovery to clinical application. Reach out to explore how our integrated sourcing model can accelerate your workflows.