Anti-CD66a/CEACAM1 Rabbit PolymAb® for WB, IHC, IF, ELISA - P13688 / P31809

Core Product Specifications and Parameters

Biological Background: CD66a/CEACAM1 Function and Localization

• CEACAM1 (CD66a) is a member of the carcinoembryonic antigen (CEA) family and functions as a homophilic, calcium-independent cell adhesion molecule.
• Acts as a coinhibitory receptor in T cells, NK cells, and neutrophils; phosphorylation-dependent PTPN6 recruitment inhibits TCR-mediated cytotoxicity, T cell proliferation, and cytokine production. Related references: PMID:18424730 PMID:23696226 PMID:25363763
• Inhibits cell migration and scattering by binding to FLNA, disrupting the FLNA-RALA interaction. Related references: PMID:16291724
• Regulates insulin homeostasis by promoting insulin clearance and modulating hepatic fatty acid synthesis via INSR-mediated phosphorylation and FASN interaction.
• Functions in angiogenesis and arteriogenesis, promoting endothelial cell differentiation, migration, and vascular remodeling post-ischemia.
• Primarily localized to the plasma membrane in multiple domains (lateral, apical, basal) and cell junctions (adherens junctions); also found in cytoplasmic vesicles, microvilli, and secreted forms.
• Expressed in colon epithelial cells, granulocytes, lymphocytes, and T cells, with alternative splicing generating isoforms with differential cytoplasmic domain lengths.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein covering human and mouse CEACAM1; cross-reactivity with both species is reported. For species-specific applications, confirm reactivity in the appropriate lysate or tissue.
• Due to alternative splicing and glycosylation, CEACAM1 exhibits multiple isoforms with molecular weights from ~30 kDa to 57 kDa. In Western blot, expect multiple bands; validate against known positive controls.
• For immunohistochemistry (IHC), colon epithelium is a suitable positive control tissue as CEACAM1 is highly expressed in columnar epithelial cells.
• In immunofluorescence (IF), the protein localizes to cell membranes and junctions; use appropriate fixation and permeabilization protocols to detect surface and intracellular pools.
• For ELISA, consider pairing with a capture antibody targeting a different epitope to avoid competition from soluble CEACAM1 forms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering this CEACAM1 antibody alongside ready-to-use antibody pairs, recombinant proteins, and ancillary reagents. Whether you need bulk antibody production or rare target sourcing, our integrated supply chain accelerates your diagnostic development from concept to clinic.