Anti-CD64 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P12314

Core Product Specifications and Parameters

Biological Background: CD64 Function and Localization

• CD64, also known as Fc-gamma RI (FcRI), Fc-gamma RIA, and FCGR1A, is a high-affinity receptor for the Fc region of immunoglobulins gamma (IgG). It is a member of the immunoglobulin superfamily and plays a pivotal role in linking humoral and cellular immunity.
• The protein functions in both innate and adaptive immune responses by mediating IgG effector functions on monocytes, triggering antibody-dependent cellular cytotoxicity (ADCC) of virus-infected cells.
• CD64 is primarily expressed on the cell membrane of monocytes and macrophages, consistent with its role in immune surveillance and phagocytosis.
• The receptor contains multiple immunoglobulin-like domains, a transmembrane helix, and a cytoplasmic tail. It undergoes post-translational modifications including glycosylation and phosphorylation, which may regulate its signaling and binding affinity.
• Alternative splicing of the FCGR1A gene produces multiple transcript variants, potentially influencing receptor function and expression.
• Keywords associated with CD64 include 3D-structure, alternative splicing, cell membrane, disulfide bond, glycoprotein, IgG-binding protein, immunity, immunoglobulin domain, innate immunity, membrane, phosphoprotein, receptor, signal, transmembrane, and reference proteome.

Experimental Guidance and Technical Tips

• For western blotting, a band around 43 kDa is expected; pre-incubating with the immunogen peptide can confirm specificity.
• The antibody has been validated in human, mouse, and rat species, making it suitable for cross-species studies; however, validation in the relevant experimental system is recommended.
• For immunofluorescence, consider using appropriate permeabilization and blocking steps to detect membrane-localized CD64 on monocytes/macrophages.
• ELISA applications may require optimization of coating buffers and detection antibodies to achieve optimal signal.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, providing validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents for immunology and infectious disease research. This CD64 rabbit pAb supports diagnostic assay development for immune function and ADCC studies. Contact us for customized sourcing solutions tailored to your clinical and research needs.