Anti-CD63 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P08962

Core Product Specifications and Parameters

Biological Background: CD63 Function and Localization

• CD63, also known as granulophysin, LAMP-3, Limp1, ME491, OMA81H, and Tspan-30, is a member of the tetraspanin superfamily, characterized by four transmembrane domains and two extracellular loops.
• It functions as a cell surface receptor for TIMP1 and plays a pivotal role in activating signaling cascades, including integrin signaling through ITGB1, leading to activation of AKT, FAK/PTK2, and MAP kinases, thereby promoting cell survival, adhesion, spreading, and migration.
• CD63 is involved in intracellular vesicular transport and is essential for normal trafficking of the PMEL luminal domain, which is critical for melanocyte development and maturation.
• It regulates leukocyte adhesion to endothelial cells by modulating SELP trafficking, and may play a role in mast cell degranulation via Ms4a2/FceRI stimulation.
• Subcellularly, CD63 is primarily located in lysosomal and late endosomal membranes, but is also found in multivesicular bodies, melanosomes, secreted extracellular exosomes, and the cell surface.
• The protein is heavily glycosylated and palmitoylated, features that are important for its trafficking and function, and it is differentially expressed in platelets, hematopoietic cells, tissue macrophages, and melanoma cells.
• Alternative splicing generates isoforms of CD63, which may have distinct functions and localizations.
• Keywords associated with CD63 include glycoprotein, lipoprotein, membrane, protein transport, and secreted, reflecting its diverse roles in cellular processes.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 103–203 of human CD63, a region that spans part of the large extracellular loop. This design may enable recognition of both the full-length protein and potential cleavage products; validate in the relevant sample system.
• For Western blot, consider that CD63 is heavily glycosylated, which may cause its apparent molecular weight to shift above the predicted 26 kDa. Ensure adequate protein deglycosylation or use positive controls to confirm band identity.
• The antibody is qualified for IHC-P and IF/ICC; optimize antigen retrieval and permeabilization conditions, especially given CD63's intracellular localization in endosomes and lysosomes.
• For ELISA, assess the antibody's performance with recombinant or native CD63 in your specific assay format, as binding may be influenced by protein conformation and post-translational modifications.
• CD63 expression is elevated in exosomes; thus, this antibody may be useful for exosome isolation or characterization studies when validated accordingly.

CamelBio: Your One-Stop Sourcing Bridge

As your premier partner in IVD raw materials, CamelBio simplifies sourcing for diagnostic manufacturers, offering validated polyclonal and monoclonal antibodies, optimized antibody pairs, and bulk ancillary reagents. For exosome research, cancer diagnostics, or tetraspanin studies centered on CD63, our global supply chain ensures rapid access to high-quality raw materials and technical support. From concept to clinic, CamelBio bridges your R&D needs with reliable, one-stop procurement solutions.