Anti-CD63 Monoclonal Antibody for WB, IHC-P, ELISA - P08962

Core Product Specifications and Parameters

Biological Background: CD63 Function and Localization

• CD63 is a member of the tetraspanin superfamily, also known as granulophysin, LAMP-3, ME491, and Tspan-30, and plays diverse roles in cell adhesion, signaling, and vesicular trafficking.
• It functions as a cell surface receptor for TIMP1, activating downstream signaling through ITGB1/integrin pathways that promote cell survival, migration, and cytoskeletal reorganization via AKT, FAK/PTK2, and MAP kinases.
• CD63 participates in VEGFA signaling by regulating the internalization of KDR/VEGFR2, and is essential for normal trafficking of PMEL luminal domain required for melanocyte development and maturation.
• The protein is localized to the cell membrane, lysosomal and late endosomal membranes, multivesicular bodies, melanosomes, and can be secreted on extracellular exosomes, reflecting its involvement in both intracellular transport and intercellular communication.
• CD63 is heavily glycosylated and palmitoylated, with an observed molecular weight ranging from 17 kDa to 25 kDa due to variable glycosylation, which is critical for its trafficking and functional interactions.
• Tissue expression is prominent in platelets, melanocytes (dysplastic nevi, primary melanomas), hematopoietic cells, and tissue macrophages, highlighting its relevance in cancer and immune cell biology.
• It contributes to leukocyte extravasation by regulating SELP trafficking to the endothelial surface, and may modulate mast cell degranulation specifically via Ms4a2/FceRI stimulation.

Experimental Guidance and Technical Tips

• For western blotting, prepare samples under reducing conditions and be aware that CD63 migrates as a diffuse band or multiple bands between 17–25 kDa due to its heterogeneous glycosylation; validate specificity with appropriate controls.
• In immunohistochemistry (IHC-P), robust antigen retrieval methods (e.g., citrate buffer, pH 6.0, heat-induced) are recommended due to the transmembrane nature of the antigen; test on known positive tissues such as melanoma or platelet-rich sections.
• For ELISA applications, pair this antibody with appropriately matched detection reagents and confirm linearity and sensitivity using recombinant CD63 standards or biological samples like cell lysates or exosome preparations.
• Since the immunogen corresponds to the large extracellular loop (amino acids 103-203), the antibody likely recognizes an epitope accessible in native and denatured conditions; consider this when designing capture or detection strategies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio acts as a one-stop procurement partner for IVD manufacturers and research labs, offering a streamlined supply of validated antibodies, bulk ancillary reagents, and rare-target raw materials. With expertise in cancer and exosome biology, CamelBio can source high-quality materials to support assay development, from monoclonal and polyclonal antibodies to recombinant proteins and technical consultation. Whether you need reproducible antibody pairs, large-scale production, or hard-to-find reagents, our global network ensures reliable delivery for every stage of your diagnostic pipeline.