Anti-CD62P/P-selectin Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P16109

Core Product Specifications and Parameters

Biological Background: CD62P/P-selectin Function and Localization

• CD62P/P-selectin (encoded by the SELP gene) is a Ca²⁺-dependent adhesion receptor belonging to the selectin family, which also includes E-selectin and L-selectin.
• It mediates the initial tethering and rolling of leukocytes on activated endothelium or platelets by binding to sialyl-Lewis X carbohydrates on glycoproteins such as PSGL-1 (SELPLG).
• P-selectin also contributes to stable cell adhesion and signaling through interactions with integrins alpha-IIb/beta3 (ITGA2B:ITGB3) and alpha-V/beta-3 (ITGAV:ITGB3). Related references: PMID:37184585
• The protein is primarily stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists like thrombin or histamine, it is rapidly translocated to the plasma membrane.
• Subcellularly, P-selectin is a single-pass type I transmembrane protein, with its N-terminal lectin domain facing the extracellular space and a short cytoplasmic tail.
• Key structural features include an N-terminal C-type lectin domain, an EGF-like domain, a series of sushi (CCP) repeats, a transmembrane helix, and a palmitoylated cytoplasmic tail.
• Post-translational modifications such as N-glycosylation, disulfide bonds, and palmitoylation are critical for proper folding, trafficking, and function.
• P-selectin plays a pivotal role in inflammation, thrombosis, and cancer metastasis, making it an important biomarker and therapeutic target.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 30–200 of human P-selectin) covers the N-terminal lectin and EGF-like domains, which are highly immunogenic and accessible for antibody binding in both native and denatured conditions.
• For Western blot (WB), the predicted molecular weight is ~91 kDa, though post-translational modifications such as glycosylation may lead to a higher apparent mass. Use reducing conditions and validate in cell lysates known to express P-selectin, such as activated platelets or endothelial cells.
• In immunohistochemistry (IHC-P), P-selectin expression is typically low in resting tissues. Consider using antigen retrieval methods and including positive controls like inflamed or thrombotic tissue sections.
• For ELISA applications, this polyclonal antibody may serve as a capture or detection reagent. Pair it with a suitable monoclonal antibody for sandwich ELISA formats to improve specificity.
• As a polyclonal antibody, lot-to-lot consistency should be verified if used in quantitative assays. Storage at -20°C in aliquots is recommended to maintain activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to IVD raw materials, from validated antibody pairs and optimized polyclonal/monoclonal antibodies to bulk ancillary reagents and rare-target sourcing. This anti-CD62P/P-selectin antibody supports inflammation and adhesion research, and is part of CamelBio's extensive catalog of high-quality raw materials for immunoassay development. Our platform streamlines procurement and technical support, helping you accelerate from concept to clinic.