Anti-CD62P/P-selectin Rabbit Monoclonal Antibody for WB, IHC-P, IF-P, ELISA - P16109

Core Product Specifications and Parameters

Biological Background: P-selectin Function and Localization

• P-selectin (SELP), also designated CD62P, GMP-140, LECAM3, and PADGEM, is a member of the selectin family of cell adhesion molecules. It is a type I transmembrane glycoprotein featuring an N-terminal C-type lectin domain, an EGF-like domain, multiple sushi (SCR) repeats, and a cytoplasmic tail.
• Functions as a Ca²⁺-dependent receptor that binds carbohydrate ligands, primarily sialyl-Lewis X, on myeloid cells such as neutrophils and monocytes. This interaction mediates the initial tethering and rolling of leukocytes on activated endothelium and platelets during inflammation.
• Through its counter-receptor SELPLG (PSGL-1) on leukocytes, P-selectin supports leukocyte rolling along vascular surfaces, a critical prerequisite for firm adhesion and extravasation.
• Additionally, P-selectin facilitates cell–cell interactions and adhesion by engaging integrin αIIb/β3 (ITGA2B:ITGB3) and αV/β3 (ITGAV:ITGB3), contributing to platelet–leukocyte aggregate formation. Related references: PMID:37184585
• Subcellularly, P-selectin is stored in the α-granules of platelets and Weibel–Palade bodies of endothelial cells. Upon cellular activation by agonists such as thrombin or histamine, it is rapidly translocated to the plasma membrane.
• Post‑translational modifications include disulfide bond formation, palmitoylation on cytoplasmic cysteine residues, and N‑linked glycosylation. Keywords: Cell adhesion, Calcium, Disulfide bond, Glycoprotein, Lipoprotein, Membrane, Metal-binding.
• The protein has a calculated molecular weight of approximately 91 kDa and may exhibit mobility shifts on SDS-PAGE due to heterogeneous glycosylation.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 42–771 of human P-selectin, corresponding to nearly the entire extracellular region. This design favors recognition of native conformations in cell‑based applications.
• For Western blotting, consider using reducing conditions and a molecular weight standard near 91 kDa. Due to glycosylation, the band may appear diffuse or migrate slightly higher.
• In immunohistochemistry (paraffin), antigen retrieval (heat‑ or enzyme‑based) is recommended; validate antibody specificity on inflamed tissue where P-selectin expression is upregulated.
• For immunofluorescence, permeabilization enables detection of both intracellular stores and surface‑translocated protein. Co‑localization with organelle markers (e.g., von Willebrand factor for Weibel–Palade bodies) can be instructive.
• As a rabbit monoclonal, this antibody is compatible with multiplex staining protocols using mouse primary antibodies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines procurement for IVD developers and research laboratories by offering a curated range of raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For projects targeting inflammation, thrombosis, or endothelial biology, this anti‑P‑selectin antibody can be integrated into assay development workflows, and CamelBio can assist in scaling supply or sourcing complementary rare‑target reagents to support your path from concept to clinic.