Anti-CD62P/P-selectin Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P16109

Core Product Specifications and Parameters

Biological Background: P-selectin Function and Localization

• P-selectin (CD62P, GMP-140, LECAM3, PADGEM) is a transmembrane adhesion molecule of the selectin family, encoded by the SELP gene.
• It functions as a Ca²⁺-dependent receptor that mediates the initial tethering and rolling of leukocytes on activated endothelium or platelets by binding to sialyl-Lewis X carbohydrates on neutrophils and monocytes.
• Through its interaction with integrins alpha-IIb/beta3 (ITGA2B:ITGB3) and alpha-V/beta-3 (ITGAV:ITGB3), P-selectin facilitates cell-cell adhesion and stable leukocyte recruitment. Related references: PMID:37184585
• P-selectin is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells and is rapidly translocated to the cell surface upon cellular activation by agonists.
• Tissue specificity is primarily in platelets and endothelial cells, where it is mobilized to the surface during inflammatory responses.
• The protein contains EGF-like and sushi domains, disulfide bonds, and palmitoylation sites, and it is classified as a lectin and calcium-binding membrane glycoprotein.
• Its molecular weight is approximately 91 kDa (UniProt: 90820 Da).

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein spanning amino acids 42–771, covering most of the extracellular region; this may allow recognition of native and recombinant P-selectin in various assay formats.
• For Western blotting, consider testing both reducing and non-reducing conditions, as P-selectin is glycosylated and may exhibit an altered apparent molecular weight.
• In immunofluorescence/immunocytochemistry (IF/ICC), appropriate permeabilization and fixation conditions should be optimized, given that P-selectin is surface-exposed upon cellular activation.
• For ELISA applications, titration of the antibody and validation with relevant standards or controls is recommended to ensure sensitivity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides one-stop access to IVD raw materials, technical services, and consulting, supporting diagnostic manufacturers, labs, and research institutes from concept to clinic. We specialize in sourcing validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, including rare target raw-material acquisition for cell adhesion and inflammation research. For projects targeting P-selectin, our team can assist in procuring high-quality antibodies and related reagents tailored to your assay development needs.