Anti-CD62P/P-selectin Monoclonal Antibody for WB, IHC-P, ELISA - P16109

Core Product Specifications and Parameters

Biological Background: CD62P/P-selectin Function and Localization

• P-selectin (CD62P), also known as GMP-140, PADGEM, and LECAM3, is a transmembrane glycoprotein encoded by the SELP gene. It belongs to the selectin family of adhesion molecules (UniProt: P16109).
• It functions as a Ca²⁺-dependent receptor that mediates the initial rolling adhesion of leukocytes on activated endothelium or platelets by binding sialyl-Lewis X carbohydrates on neutrophils and monocytes.
• P-selectin mediates cell-cell interactions and cell adhesion via interaction with integrin alpha-IIb/beta3 (ITGA2B:ITGB3) and integrin alpha-V/beta-3 (ITGAV:ITGB3). Related references: PMID:37184585
• The protein is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells, and is rapidly translocated to the cell surface upon activation by agonists such as thrombin or histamine.
• Structurally, P-selectin contains an N-terminal lectin domain, an EGF-like domain, multiple Sushi (SCR) repeats, a transmembrane helix, and a short cytoplasmic tail. It is modified by palmitoylation and disulfide bond formation.
• Key biological roles highlighted by UniProt keywords: cell adhesion, calcium binding, lectin activity, and involvement in the inflammatory response.

Experimental Guidance and Technical Tips

• The immunogen corresponds to residues 731-830 of human P-selectin (P16109), suggesting the antibody likely targets an epitope in the C-terminal region. Consider this when designing complementary detection tools.
• For Western blot, the predicted molecular weight is approximately 91 kDa. Validate using positive controls such as activated platelet or endothelial cell lysates, and optimize antibody dilution empirically.
• In IHC-P, antigen retrieval may be required due to the transmembrane nature of the target. Use appropriate tissue sections known to express P-selectin (e.g., inflamed vascular endothelium).
• For ELISA applications, consider using recombinant P-selectin protein or cell-based assays. Pairing with a detection antibody targeting a different epitope may improve assay performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This CD62P/P-selectin antibody supports inflammation and cell adhesion research, and our team can assist with sourcing validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw materials tailored to your diagnostic needs.