Anti-CD62L/L-Selectin Polyclonal Antibody for WB, IHC-P, ELISA - P14151

Core Product Specifications and Parameters

Biological Background: CD62L/L-Selectin Function and Localization

• L-selectin, also known as CD62L, LEU8, LAM-1, and LYAM1, is a cell adhesion molecule encoded by the SELL gene.
• It functions as a calcium-dependent lectin that mediates lymphocyte adhesion to high endothelial venules (HEVs) in peripheral lymph nodes, a critical step in lymphocyte homing. Related references: PMID:12403782 PMID:28011641 PMID:28489325
• L-selectin promotes the initial tethering and rolling of leukocytes on activated endothelium, facilitating their extravasation to sites of inflammation. Related references: PMID:12403782 PMID:28011641
• The protein is localized to the cell membrane and contains an extracellular region with a C-type lectin domain, an EGF-like domain, and two sushi (CCP/SCR) domains.
• L-selectin is expressed on most peripheral blood B cells and T lymphocytes, as well as on monocytes and granulocytes.
• Post-translational modifications include N-linked glycosylation and disulfide bond formation, which are essential for proper folding and function.
• The mature protein has a predicted molecular weight of approximately 42 kDa.

Experimental Guidance and Technical Tips

• For Western blot, expect a band at approximately 42 kDa under reducing conditions; validate specificity using a positive control such as human lymphocyte lysate.
• In immunohistochemistry (paraffin-embedded), antigen retrieval methods (e.g., citrate buffer pH 6.0) may be necessary; test on lymphoid tissues like lymph node or tonsil sections.
• For ELISA applications, this polyclonal antibody can be used for either antigen capture or detection; optimize coating concentration and buffer conditions empirically.
• The immunogen corresponds to the N-terminal region (aa 1-100) of human L-selectin; confirm sequence identity if using with mouse or rat samples to ensure epitope conservation.
• Due to the polyclonal nature, lot-to-lot variability may occur; we recommend including internal controls and validating each new lot in your assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated supplier of high-quality IVD raw materials, offering a comprehensive portfolio that includes this anti-CD62L/L-Selectin polyclonal antibody, along with validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. Whether you are developing assays for lymphocyte homing, inflammation, or immune cell profiling, we provide one-stop access to reliable reagents and technical support to accelerate your diagnostic development. Our sourcing capabilities extend to rare and custom targets, ensuring you have the critical materials needed from concept to clinic.