Anti-CD62L/L-Selectin Monoclonal Antibody (APC/Cyanine7) for Flow Cytometry - P14151

Core Product Specifications and Parameters

Biological Background: L-Selectin Function and Localization

• L-selectin (CD62L, LAM-1, Leu-8, LECAM1, gp90-MEL) is a calcium-dependent (C-type) lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells. Related references: PMID:12403782 PMID:28011641 PMID:28489325
• It mediates the adherence of lymphocytes to specialized endothelial cells of high endothelial venules (HEVs) in peripheral lymph nodes, a process essential for lymphocyte homing.
• L-selectin promotes the initial tethering and rolling of leukocytes on endothelia, a critical early step in the inflammatory response. Related references: PMID:12403782 PMID:28011641
• The protein is localized to the cell membrane and is a type I transmembrane protein with an extracellular region containing an N-terminal C-type lectin domain, an EGF-like domain, and two sushi (SCR) domains.
• It is predominantly expressed in B-cell lines and T-lymphocytes, in line with its role in adaptive immune surveillance.
• L-selectin is a glycoprotein with a molecular weight of approximately 42 kDa (UniProt: 42187 Da) and is subject to alternative initiation.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular region (aa 39-332), which includes the lectin and EGF-like domains; this design may favor recognition of the native conformation and functional epitopes relevant for flow cytometry.
• For flow cytometry, use live, unfixed leukocytes or lymphocyte cell lines (e.g., Jurkat, Raji) to preserve surface CD62L expression; avoid fixation methods that may mask the epitope.
• Titrate the antibody to determine the optimal concentration for your specific cell type and experimental conditions, as surface antigen density can vary with activation state.
• Include appropriate isotype controls and consider co-staining with other lymphocyte markers (e.g., CD3, CD19) to discriminate T and B cell subsets.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. Our portfolio includes validated monoclonal antibodies like this APC/Cyanine7 anti-CD62L clone, along with matched antibody pairs, bulk ancillary reagents, and support for rare target sourcing to accelerate your immunology and adhesion research. Partner with CamelBio for end-to-end supply solutions from concept to clinic.