Anti-CD62E Rabbit Monoclonal Antibody for WB, IF/ICC, FC, ELISA - Q00690

Core Product Specifications and Parameters

Biological Background: CD62E Function and Localization

• E-selectin (CD62E/ELAM-1/LECAM2) is a cell-surface glycoprotein encoded by the Sele gene in mice.
• It mediates the adhesion of blood neutrophils to cytokine-activated endothelium by interacting with SELPLG/PSGL1. Related references: PMID:1375914
• Belongs to the selectin family, characterized by an N-terminal lectin domain, an EGF-like domain, and multiple Sushi (SCR) repeats.
• Localized to the cell membrane as a single-pass type I membrane protein.
• Contains structural features including disulfide bonds, a calcium-dependent lectin domain, and N-glycosylation sites.
• May also contribute to capillary morphogenesis.
• Molecular weight is approximately 67 kDa (calculated) or 66 kDa (observed).

Experimental Guidance and Technical Tips

• The immunogen covers the entire extracellular region (residues 22–557), which contains the lectin, EGF-like, and Sushi domains; it may be effective for recognizing both membrane-bound and shed soluble forms.
• For western blot, a specific band at the expected molecular weight (~66 kDa) should be observed; optimization of blocking and antibody dilution is recommended.
• For immunofluorescence and immunocytochemistry, fixation and permeabilization conditions should be optimized, as the target is a transmembrane protein.
• For flow cytometry, using a live-cell staining protocol is suggested to detect surface expression; include appropriate isotype controls.
• For ELISA applications, the antibody can potentially be employed as a capture or detection reagent; pairing with another clone may enhance sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD manufacturers and researchers, offering validated monoclonal antibodies, antigen-grade proteins, and ancillary reagents for E-selectin/CD62E studies. Whether you require bulk quantities, custom conjugation, or matched antibody pairs for ELISA development, our supply chain connects you with quality-controlled raw materials to accelerate your assay development.