Anti-CD59 Rabbit Monoclonal Antibody for WB, ELISA - P13987

Core Product Specifications and Parameters

Biological Background: CD59 Function and Localization

• CD59 glycoprotein, also known as protectin, HRF20, MACIF, or MIRL, is a GPI-anchored cell surface glycoprotein primarily expressed on human cells to protect against complement-mediated lysis.
• Functions as a potent inhibitor of the complement membrane attack complex (MAC), preventing damage during complement activation by blocking the final steps of pore formation. Related references: PMID:11882685 PMID:1698710 PMID:2475111
• Mechanistically, CD59 binds to the beta-hairpin regions of C8α and C8β components of the assembling MAC, forming an intermolecular beta-sheet that prevents incorporation of multiple C9 molecules necessary for osmolytic pore formation. Related references: PMID:11882685 PMID:1698710 PMID:36797260
• A soluble form of CD59, found in urine and other body fluids, retains complement binding activity but exhibits greatly reduced MAC inhibitory capacity compared to the membrane-bound form.
• Subcellularly, CD59 is anchored to the cell membrane via a GPI moiety and can also be found in secreted forms.
• The protein contains key structural features including multiple disulfide bonds and glycation sites, contributing to its stability and function.
• Genetic variants in CD59 are associated with hereditary hemolytic anemia, highlighting its critical role in protecting erythrocytes from complement attack.
• Alternative splicing produces multiple isoforms of CD59, potentially modulating its function or localization.

Experimental Guidance and Technical Tips

• The immunogen for this antibody is a synthetic peptide spanning amino acids 49–128 of human CD59, targeting an extracellular region, making it suitable for detecting the native membrane-bound form.
• For Western Blot, a major band at approximately 14 kDa is expected for the unglycosylated peptide backbone; however, glycosylation and GPI-anchor addition may result in higher molecular weight species, so validate with appropriate positive controls (e.g., human serum or erythrocyte membranes).
• In ELISA applications, consider pairing this monoclonal antibody with a different clone targeting a distinct epitope to develop a sensitive sandwich immunoassay for CD59 quantification.
• Always optimize antibody dilution and experimental conditions in the relevant sample matrix to ensure specificity and minimize background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive sourcing solution for IVD raw materials, providing diagnostic manufacturers and researchers with reliable access to validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. This anti-CD59 rabbit monoclonal antibody is part of our catalog designed for complement research and clinical assay development. Whether you need rare target raw materials or end-to-end technical support from concept to clinic, CamelBio is your trusted partner in streamlined procurement.