Anti-CD59 Polyclonal Antibody for WB, ELISA - P13987

Core Product Specifications and Parameters

Biological Background: CD59 Function and Localization

• CD59 glycoprotein (also known as protectin, HRF20, MACIF, MIRL, MAC-IP) is a cell surface inhibitor of the complement membrane attack complex (MAC).
• It potently inhibits MAC assembly by binding to the C8 components (C8A and C8B) and preventing incorporation of C9 into the forming pore, thus protecting human cells from complement-mediated lysis. Related references: PMID:11882685 PMID:1698710 PMID:2475111 PMID:36797260
• A soluble form of CD59, found in urine, retains complement binding but exhibits greatly reduced MAC inhibitory activity on cell membranes.
• CD59 is anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage and is also detectable as a secreted protein.
• The protein undergoes multiple post-translational modifications, including N-linked glycosylation, disulfide bond formation, and glycation, which contribute to its structural stability and function.
• Genetic deficiency of CD59 is associated with hereditary hemolytic anemia, underscoring its critical role in protecting erythrocytes from complement attack.
• The CD59 gene undergoes alternative splicing, yielding multiple transcript variants that may contribute to functional diversity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the mature CD59 extracellular domain (aa 26–128), suggesting that the antibody primarily recognizes the native, GPI-anchored form on cell surfaces and soluble forms lacking the GPI anchor.
• For Western blot analysis of human cell or tissue lysates, a band at approximately 14 kDa is anticipated; however, N-glycosylation may result in a broadened band or a slightly higher apparent molecular weight. Consider using reducing conditions and validating with appropriate controls.
• In ELISA applications, this polyclonal antibody can be used for either antigen capture or detection of CD59 in human serum, plasma, or recombinant protein preparations. Titration experiments (e.g., 1–5 µg/mL coating) are recommended to optimize signal-to-noise ratios.
• As a polyclonal reagent, confirm species cross-reactivity experimentally if studying non-human models, and include appropriate negative controls to account for any nonspecific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated polyclonal and monoclonal antibodies, antigen pairs, and ancillary reagents. For complement system targets such as CD59, we offer sourcing solutions for rare-antibody raw materials to streamline your assay development from concept to clinic. Contact us to discuss bulk procurement, technical consultation, or tailored supply chain management for your CD59-based diagnostic kits.