Anti-CD58 Rabbit Polyclonal Antibody for WB, ELISA - P19256

Core Product Specifications and Parameters

Biological Background: CD58 Function and Localization

• CD58 (Lymphocyte function-associated antigen 3), also known as LFA-3, is a surface glycoprotein that serves as a ligand for the T-lymphocyte CD2 glycoprotein.
• The interaction between CD58 and CD2 mediates thymocyte interactions with thymic epithelial cells, as well as antigen-independent and -dependent T-cell interactions with target cells and antigen-presenting cells.
• CD58 plays a critical role in T-lymphocyte rosetting with erythrocytes, a phenomenon used in early diagnostic assays for T-cell enumeration.
• It is a member of the immunoglobulin superfamily, containing Ig-like domains, and is anchored to the cell membrane via a single transmembrane helix.
• Post-translational modifications include N-linked glycosylation and disulfide bond formation, which are essential for its structural stability and function.
• Alternative splicing generates multiple isoforms, although the functional significance of these variants remains to be fully characterized.
• Subcellular localization is primarily at the cell membrane, consistent with its role in intercellular adhesion and signaling.

Experimental Guidance and Technical Tips

• In Western blot, expect a band around 28–40 kDa due to glycosylation; consider validating in the relevant sample system using appropriate molecular weight markers.
• For ELISA applications, consider pairing this antibody with a CD2 detection reagent to study CD58-CD2 ligand-receptor interactions.
• Ensure sample compatibility: this antibody has been validated for human CD58; cross-reactivity with other species has not been tested.
• As a polyclonal antibody, it may recognize multiple epitopes, offering robust signal but requiring careful titration for optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, including validated antibodies, antigens, and ancillary reagents. Our CD58 rabbit polyclonal antibody aligns with immunoassay development needs, offering reliable performance for WB and ELISA applications in T-cell biology. With a broad network of raw-material sources, CamelBio can also support the sourcing of rare target reagents and bulk quantities to accelerate your project from concept to clinic.