Anti-CD58 (LFA-3) Rabbit Monoclonal Antibody for WB, ELISA - P19256

Core Product Specifications and Parameters

Biological Background: CD58 Function and Localization

• CD58, also known as lymphocyte function-associated antigen 3 (LFA-3) or ag3, is a surface glycoprotein encoded by the CD58 gene.
• Acts as a ligand for the CD2 glycoprotein on T-lymphocytes, mediating cell-cell adhesion crucial for T-cell activation and function.
• Plays a key role in thymocyte interaction with thymic epithelial cells, supporting T-cell development and selection.
• Facilitates antigen-independent and -dependent interactions between T-lymphocytes, target cells, and antigen-presenting cells, enhancing immune synapse formation.
• Mediates T-lymphocyte rosetting with erythrocytes, a classic test for T-cell function.
• The LFA-3/CD2 interaction can prime responses in both CD2+ and LFA-3+ cells, indicating bidirectional signaling.
• Subcellular localization: predominantly expressed on the cell membrane as a type I transmembrane protein.
• Structural features include an immunoglobulin domain, disulfide bond(s), and a transmembrane helix; alternative splicing generates isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 29-215), suggesting the antibody likely targets the N-terminal region responsible for CD2 binding. Validate epitope specificity in your experimental system.
• For Western blot, the antibody detects a band at ~28 kDa under reducing conditions. Optimize protein extraction and loading controls for your cell type.
• ELISA protocols should use the antibody as a capture or detection reagent; consider pairing with another anti-CD58 clone for sandwich ELISA if sensitivity is required.
• Confirm cross-reactivity with non-human primate or other species if needed; validation data currently supports human reactivity.
• For immunohistochemistry or flow cytometry, test the antibody's performance under native conditions; the epitope is in the extracellular domain, so it may work for live-cell staining.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with comprehensive access to IVD raw materials, including validated antibody pairs, high-quality monoclonal antibodies, and ancillary reagents. This anti-CD58 rabbit mAb supports immunology and T-cell research and is part of a broader portfolio of antibodies for cell surface markers. For custom assay development or bulk orders, CamelBio's one-stop sourcing model streamlines procurement from discovery to regulatory submission.